2018年1月21日 · 该信号通路的七个基因编码两组分调节蛋白PhoB和PhoR，以及高亲和力磷酸盐转运蛋白PstSCAB和辅助蛋白PhoU。 由于传感器激酶PhoR没有周质感觉域，因此这些细胞感知环境磷酸盐的机制尚不清楚。 本文探讨了以下假设：PstSCAB转运蛋白的交替构象是正常磷酸盐转运周期的一部分，表明磷酸酯充足或磷酸盐限制。 结果我们测试了PstB的两个变异体，预测它们的信号输出可将蛋白质锁定在两种构象中的一种。 我们观察到，pstBQ160K突变体预计位于向内 …

In this paper, we test this model by creating pstB mutations that are in the same positions as the Q140K and E150Q malK mutations described above that are predicted to favor stable inward-facing and outward-facing conformations of the transporter and then assay the signaling states of the system. Methods Strains, plasmids and media

2019年8月3日 · PstB蛋白是位于胞内的ATP结合蛋白，其实质上是一个ATP酶，在磷的转运过程中，其主要作用是结合并水解ATP，通过能量变化来促进或抑制Pst系统对Pi的摄取和转运。PstB蛋白具有保守的核苷酸结合位点，由pstB基因编码，也受双组分

2005年6月30日 · The nucleotide binding subunit of the phosphate-specific transporter (PstB) from Mycobacterium tuberculosis is a member of the ABC family of permeases, which provides energy for transport through ATP hydrolysis. We utilized the intrinsic fluorescence of the single tryptophan containing protein to study the structural and conformational changes ...

2001年11月30日 · The B-subunit of phosphate-specific transporter (PstB) is an ABC protein. pstB was polymerase chain reaction-amplified from Mycobacterium tuberculosis and overexpressed in Escherichia coli. The overexpressed protein was found to be in inclusion bodies.

2017年7月11日 · Here, we demonstrate that a phosphate-sensing signal transduction system, comprising the Pst phosphate transporter, the two-component system SenX3-RegX3, and functionally redundant PhoY proteins that mediate signaling between Pst and SenX3-RegX3, influences persister formation.

2016年10月14日 · Our investigations reveal the presence of multiple Pst phosphate uptake systems that exist across three distinct operons in Nostoc punctiforme and functionally characterize the role of the gene product PstB1 as being crucial for …

To understand the mechanisms that facilitate phosphate accumulation in the cyanobacteriumNostoc punctiforme, phosphate transport systems were identified, revealing a redundancy of Pst phosphate uptake systems that exists across three distinct operons.

2016年8月24日 · Our data indicate that the two M. tuberculosis Pst transporters have distinct physiological functions, with the PstA1 transporter being specifically involved in phosphate sensing and gene regulation while the PstA2 transporter influences survival in acidic conditions.

Genetic evidence suggests that the intergenic region between pstA and pstB plays a role in the regulation of rpoS translation during phosphate limitation. In addition to the Pho regulon, phosphate starvation also stimulates the accumulation of RpoS.