Cryptococcus neoformans CAP59 (or Cap59p) is involved in the
Our results suggest that CAP59 is involved in capsule synthesis by participating in the process of GXM (polysaccharide) export. Several genes are essential for Cryptococcus neoformans capsule synthesis, but their functions are unknown. We examined the localization of glucuronoxylomannan (GXM) in strain B-3501 and in cap59 mutants B-4131 and C536.
Further analysis of the CAP59 locus of Cryptococcus neoformans ...
Dec 29, 1995 · Cryptococcus neoformans (Cn) produces an extracellular polysaccharide capsule that is an essential factor for virulence. We previously isolated a gene, CAP59, which is necessary for capsule formation. To dissect the functional region of CAP59, we placed it under control of the Cn GAL7 promoter ( pGAL7 ).
Isolation of the Third Capsule-Associated Gene,
DNA sequence analysis revealed that CAP60 has similarity to CAP59 at the center portion of its coding regions. Contour-clamped homogeneous electric field blot analysis suggested that these two genes are on the same chromosome.
Capsule-associated genes of serotypes of - Oxford Academic
Mar 1, 2006 · In this study, the nucleotide sequences of the capsule-associated genes CAP10, CAP59, CAP60 and CAP64 from five serotypes of C. neoformans were analyzed for their phylogenetic relationships, with special regard to serotype AD. The nucleotide sequence analyses showed that serotype AD had two different sequences in these four genes.
Sequence length required for homologous recombination in …
Feb 1, 2003 · The blot was probed with a fragment of the CAP59 gene that hybridizes to a ∼3.0 kb band in the parental H99 and to a larger, ∼5.5 band in the disruptants. There was perfect correlation between the PCR results and the Southern blot data ( Fig. 2C and data not shown).
Complementation of a capsule-deficient mutation of ... - PubMed
When the capsule-deficient phenotype was complemented, the originally avirulent C. neoformans strain became virulent for mice. In addition, the acapsular strain created by gene deletion of CAP59 lost its virulence. This work demonstrates the molecular basis for capsule-related virulence and that the CAP59 gene is required for capsule formation.
(PDF) Isolation of the Third Capsule-Associated Gene
May 1, 1998 · DNA sequence analysis revealed that CAP60 has similarity to CAP59 at the center portion of its coding regions. Contour-clamped homogeneous electric field blot analysis suggested that these two...
Cryptococcus neoformans CAP59 (or Cap59p) Is Involved in the ...
Apr 1, 2004 · Our results suggest that CAP59 is involved in capsule synthesis by participating in the process of GXM (polysaccharide) export. Cryptococcus neoformans is the etiologic agent for cryptococcosis, a major opportunistic mycosis in patients with AIDS (23).
Dot blot analysis of whole cells from the C. neoformans wild type …
Cap59 has been suggested to play a role in extracellular trafficking of multimeric forms of GXM molecules (26). Moreover, it may play a role in the assembly of GXM, since it shares homology with...
Further analysis of the CAP59 locus of Cryptococcus neoformans ...
Dec 29, 1995 · Cryptococcus neoformans (Cn) produces an extracellular polysaccharide capsule that is an essential factor for virulence. We previously isolated a gene, CAP59, which is necessary for capsule formation. To dissect the functional region of CAP59, we placed it under control of the Cn GAL7 promoter (pGAL7).