Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with excitation and emission wavelengths of 495 and 515 nm, respectively, and has the appearance of orange crystals. Calcein self- quenches at concentrations above 70 mM and is commonly used as an indicator of lipid vesicle leakage.

Calcein AM is a cell-permeant dye frequently used in cell biology research. It is derived from Calcein, a fluorescent dye that exhibits bright green fluorescence upon binding to intracellular free calcium ions. Calcein AM is a non-fluorescent compound that can permeate the cell membrane due to its lipophilic properties.

Calcein AM staining is a commonly used technique for live cell imaging, utilizing the non-fluorescent acetoxymethyl (AM) ester form of Calcein. This compound readily crosses live cell membranes due to its neutral charge.

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Calcein-AM is a cell-permeable esterase substrate that produces the highly fluorescent dye calcein after intracellular esterase hydrolysis in live cells.

Calcein has been extensively used to investigate endosomal escape of various types of nanocarriers within cells. However, the performed methods show differences in several aspects which demonstrates the complexity as well as the robustness of the assay.

Calcein may be used as a specific immunohistochemical marker for the identification of ameloblastomas. At present, the biological function of calcein is unknown; it may be related to the occurrence, development, and invasion of ameloblastoma, but the specific mechanism needs to be further explored.

2024年9月13日 · Calcein AM is a green fluorogenic dye that stains live cells for cell viability in flow cytometry and fluorescence microscopy. It is the acetoxymethyl (AM) ester form of the fluorescent probe, calcein.

The calcein AM (calcein-acetoxymethyl ester) assay is a widely used fluorescent technique for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance. When cells in culture are exposed to calcein AM, it passes the plasma membrane and is hydrolyzed by cytosolic unspecific esterases to become fluorescent calcein in living cells.