Under the chosen HPLC conditions, caffeine eluted at 6.7 minutes and an overlay of the injected concentrations from each calibration shows good peak shapes for all concentrations and retention time conformance (Figure 2).

We developed a rapid and sensitive analytical method for the simultaneous determination of caffeine and its three principal metabolites in adult and newborn human plasma using HPLC technique after solid-phase extraction with Strata-X™ plates.

A simple, accurate, and sensitive high-performance liquid chromatography (HPLC) method for caffeine from Coffea arabica has been developed and validated. The developed HPLC method was validated for linearity, specificity, precision, recovery, limits of detection, and limits of quantification by the International Conference on Harmonization ...

2016年12月19日 · A simple HPLC-UV method was developed and validated for the determination of Caffeine content in tea and coffee samples of different local brands commercially available at Kathmandu and Kaski,...

2012年9月1日 · Here in this review we have summarized various HPLC methods used for the caffeine analysis in various samples and complex mixtures with their chromatographic column, mobile phase, flow rate ...

A simple HPLC-UV method was developed and validated for the determination of Caffeine. Standard caffeine was separated through c-18 column (YMC ODS 5μm, internal diameter 4.6 nm and length 250 mm) using methanol and water (80:20) as a mobile

2025年1月6日 · Caffeine, a commonly recognized compound, plays a vital role in HPLC calibration for several reasons. This article delves into the characteristics and benefits of using caffeine as a standard in HPLC calibration, while also highlighting its practical applications.

This SOP uses an HPLC to quantify the caffeine concentration in generic brewed coffee by assaying a series of caffeine standards in order to construct a calibration curve, and then assaying coffee samples and calculating caffeine concentration against the calibration curve.

Qualitative analysis of caffeine content with HPLC was done by comparing the retention time of caffeine standard (pure compound) with the sample. From the experiment, the average standard caffeine tR was 2.518 minutes and the mean t R of X, Y, and Z samples were 2.508 minutes, 2.491 minutes, and 2.495 minutes, respectively.

The peak area of the component of interest (caffeine) will then give us a quantified mg/ml value for each sample tested through the use of a calibration curve.