Chromatin assembly factor-1 (CAF-1) is a protein complex — including Chaf1a (p150), Chaf1b (p60), and p48 subunits in humans, or Cac1, Cac2, and Cac3, respectively, in yeast— that assembles histone tetramers onto replicating DNA during the S phase of the cell cycle.

2023年8月25日 · 染色质组装因子CAF-1是在进化过程中保守的异源三聚体组蛋白伴侣复合物，负责将新合成的H3-H4组蛋白装配到子链DNA上，完成从头装配的核小体组装的第一步，即形成一个由DNA缠绕H3-H4四聚体组成的核小体组装中间态Tetrasome（四聚小体）。

染色质遗传需要染色质组装因子-1 (caf-1)在dna复制后重新组装核小体。然而，关于caf-1的组蛋白结合模式和核小体组装过程的直接知识是缺乏的。

Chromatin assembly factor-1 (CAF-1) is an evolutionarily conserved heterotrimeric protein complex responsible for the deposition of newly synthesized histones H3 and H4 onto DNA. A mechanistic understanding of the structure and function of CAF-1 is needed to fully comprehend the principles of chromatin inheritance.

2015年12月9日 · Together, our findings reveal the histone chaperone CAF-1 to be a novel regulator of somatic cell identity during transcription-factor-induced cell-fate transitions and provide a potential...

Here, we review the biology of the CAF-1 complex, with an emphasis on CHAF1b, including its structure, regulation, and function. In addition, we discuss the possible contributions of CHAF1b and the CAF-1 complex to human diseases. Of note, CHAF1b is located within the Down syndrome critical region (DSCR) of chromosome 21.

染色质组装因子1（chromatin assembly factor-1，CAF-1），是由p150、p60、p48三个亚单位组成的组蛋白伴侣。CAF-1主要功能是在DNA复制中与增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）相互作用，负责募集组蛋白H3、H4沉积在新合成的DNA上以促进核小体装配。

2022年4月29日 · We show that CAF-1 suppression triggers rapid differentiation of myeloid stem and progenitor cells into a mixed lineage state. We find that CAF-1 sustains lineage fidelity by controlling...

Here, we review the biology of the CAF-1 complex, with an emphasis on CHAF1b, including its structure, regulation, and function. In addition, we discuss the possible contributions of CHAF1b and the CAF-1 complex to human diseases. Of note, CHAF1b is located within the Down syndrome critical region (DSCR) of chromosome 21.

2016年5月25日 · Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that...