Caco-2 [Caco2] is an adherent cell line isolated from colon tissue derived from a patient with colorectal adenocarcinoma. These cells express characteristics of enterocytic differentiation upon confluence and are a commonly used line for mimicking the small intestine in …

The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with …

Although the colonic cell line Caco-2 is widely used as a model of the small intestinal barrier function, it has limitations such as overestimated transepithelial electrical resistance (TEER) compared to in vivo conditions.

The Caco-2 cell line derived from human colon carcinoma is commonly used to assess the permeability of compounds in in vitro conditions. Due to the significant increase in permeability studies using the Caco-2 cell line in recent years, the need to ...

The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with …

Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96:736-749(1989) PubMed= 7764660 ; DOI= 10.1007/BF00749813

Caco-2, a cell line obtained from human colon adenocarcinoma, is the most well-established intestinal cell model to date. Caco-2 cells differentiate into enterocyte-like cells after 21 days in culture and express a number of characteristic transporters [1,2]. Since Caco-2 is the gold-standard model to study intestinal absorption, this review ...

The Caco-2 cell line is instrumental in understanding the function and molecular details of intestinal epithelium efflux systems, such as P-glycoprotein, crucial for drug development.

2025年1月10日 · The Caco-2 cell model has been widely used to assess the intestinal permeability of drug candidates in vitro, owing to its morphological and functional similarity to human enterocytes. While Caco-2 cell assay is considered safe and cost-effective, it is also characterized by being time-consuming.

2022年8月25日 · The Caco-2 monolayer is regarded as an in vitro gold-standard in prediction of intestinal permeability. It finds extensive use in preclinical studies for screening of drugs and elucidating their transport mechanisms, presystemic metabolism and cytotoxicity.