High levels of AAV vector integration into CRISPR-induced DNA …
2019年9月30日 · A miniaturized AAV allows characterization of CRISPR DSBs. The profile of AAV integration in CRISPR cut sites determined by NGS appears to favor the ITR region.
AAV-based CRISPR-Cas9 genome editing: Challenges and …
2024年3月1日 · This review discusses current challenges in delivering CRISPR-Cas nuclease-based genome editing therapies using AAV and highlights emerging methods to overcome …
An AAV-CRISPR/Cas9 strategy for gene editing across divergent …
2023年5月31日 · We adopted an adeno-associated virus (AAV)–based strategy to deliver the CRISPR/Cas9 components to adult neural tissue and reduce OXTR protein levels in vivo. The …
Self-inactivating, all-in-one AAV vectors for precision Cas9 ... - Nature
2021年11月1日 · Here, we describe single-vector, ~4.8-kb AAV platforms that express Nme2Cas9 and either two sgRNAs for segmental deletions, or a single sgRNA with a homology-directed …
The AAVantages of AAV in CRISPR Screens - blog.addgene.org
2025年2月4日 · Additionally, while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when …
Delivery of CRISPR/Cas9 system by AAV as vectors for gene therapy
2024年11月15日 · This review delineates the mechanism of the AAV delivery CRISPR/cas9 system to accomplish gene editing, elucidates its application in disease treatment, …
Peptide-enabled ribonucleoprotein delivery for CRISPR ... - Nature
4 天之前 · The use of AAV in conjunction with electroporation-based delivery of CRISPR RNP has been shown to impair the capacity for HSPCs to engraft 22 and might have contributed to …
Viral Delivery Systems for CRISPR - PMC - PubMed Central (PMC)
A large number of AAV serotypes also allow for tissue-targeted gene delivery, as various serotypes have been shown to be suitable for CRISPR-editing in specific tissues (e.g., AAV9 …
A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing
Our self-deleting AAV-CRISPR system establishes critical proof of concept that transient expression of Cas9 with AAV vectors can be achieved, capitalizing on the unparalleled …
High levels of AAV vector integration into CRISPR-induced …
2019年9月30日 · The integration profile of AAV-465λ in cultured cells display both full-length and fragmented AAV genomes at Cas9 on-target sites. Our data indicate that AAV integration …
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