2021年5月17日 · CK2 is a constitutively active Ser/Thr protein kinase, which phosphorylates hundreds of substrates, controls several signaling pathways, and is implicated in a plethora...

2023年8月22日 · Here, we show CK2α, a Wnt/β-catenin signaling Ser/Thr kinase, phosphorylates Daam2, inhibiting its function and Wnt activity during OL development. Intriguingly, we found Daam2 phosphorylation differentially impacts distinct stages of OL development, accelerating early differentiation followed by decelerating maturation and myelination.

Our study is the first report that CK2 activity is regulated acutely in response to stimulation of Wnt3a. We have demonstrated that Wnt stimulates transient activation of CK2 and that the CK2 response is obligate for Wnt-stimulated accumulation of β-catenin and activation of Lef/Tcf-dependent transcription in canonical pathway .

2021年3月9日 · Alternatively, by developing a method to isolate intact condensates, they identify Casein Kinase 2 (CK2) as being detected within DACT1 condensates. CK2 has been previously shown to interact...

2006年11月21日 · The finding that CK2 phosphorylates LEF-1 and enhances binding to β-catenin, while blocking binding to TLE1, indicated that it might regulate the assembly of active Wnt enhancers. In this respect, CK2 would function oppositely to CK1, which promotes binding of β-catenin to the APC tumor suppressor, disfavors binding to LEF-1/TCF proteins 16 ...

We have shown that overexpression of CK2 catalytic subunits is capable of promoting tumorigenesis, and that loss of CK2 catalytic subunits in development can be lethal. Through studies in cells, mice, and frogs, we and others have identified the Wnt and NF-κB pathways as two key signal transduction pathways that are regulated by CK2 activity ...

A notable pathway of the CK2 is Wnt-signaling, as it binds directly to both β-catenin and T cell-specific transcription factor/lymphoid enhancer-binding factor (TCF/LEF) [17,48,49,50,51]. Furthermore, CK2 phosphorylates other substrates such as dishevelled (Dvl) and adenomatous polyposis coli (APC) [ 17 , 52 , 53 ].

Through studies in cells, mice, and frogs, we and others have identified the Wnt and NF-kappaB pathways as two key signal transduction pathways that are regulated by CK2 activity, in embryonic development and in cancer. These results suggest that inhibiting CK2 could be useful in treating cancer, but dangerous to developing organisms.

Our studies suggest that phosphorylation of critical oncogenes by CK2, as well as by other serine-threonine kinases, regulates their stability via susceptibility to the proteasomal degradation system. Beta-catenin is a transcriptional co-factor in the …

The second branch of the non-canonical pathway – WNT/Ca 2+ signaling is characterized by WNT-Fz-induced phospholipase C (PLC) activation that increases cytoplasmic Ca 2+ levels. Several Ca 2+ -responsive enzymes such as protein kinase C (PKC), calcineurin, and calcium/calmodulin-dependent protein kinase II (CaMKII) are activated upon sensing ...