2022年8月13日 · Epidermal CD147-overexpression or knockout (Epi CD147-OE or Epi CD147-KO) transgenic mouse models were generated for in vivo study. RNA-sequencing and q-PCR were performed to identify the differentially expressed genes.

2020年9月23日 · We report that T and NK cells transduced with a CAR that recognizes the surface marker, CD147, also known as Basigin, can effectively kill various malignant HCC cell lines in vitro, and HCC...

2022年6月8日 · We now confirmed CD147 as miR-146a direct target gene and demonstrate that functional CD147-MCT1 transmembrane complexes are necessary for cellular metabolism supporting tumor growth,...

2024年6月16日 · We aimed to engineer CD147 KO THP-1 cells and evaluated their differentiation properties compared to the wild type. A humanized anti-CD147 antibody, HuM6-1B9, was also constructed for investing the phagocytic function of CD147 KO THP-1 cells mediated by HuM6-1B9 in the phagocytosis of Jurkat T cells.

2021年6月30日 · To understand whether CD147 can be used as a therapeutic target for oral cancer, CRISPR/Cas9 gene-editing technology was used to knock out the CD147 gene in cal27 cells to obtain knockout cell lines. Using CCK-8, Transwell, RT-PCR, and Western blotting, the proliferation and invasion abilities of the knockout cell lines were decreased ...

2017年3月28日 · In this study, we analyzed thymocyte development in a mouse strain that carries the conditional CD147 allele and transgenic Cre recombinase under the control of the proximal Lck promoter (CD147 T-KO mice (Yao et al., 2013)). Using this model, we made a surprising discovery that the loss of thymic cellularity and diminished cells of the T-cell ...

Two CD147 knockout (CD147 KO) CCA cell (KKU-213) clones were established using the CRISPR/Cas9 system. Cell migration and invasion were determined using a Boyden chamber assay. Temporal protein levels were modified by siRNA, specific inhibitors and/or activators.

cd147作为一个多功能的跨膜糖蛋白，高表达在多种肿瘤细胞表面，促进肿瘤的侵袭和转移。在肺癌和肝癌治疗中，cd147特异性抗体被证明是有效的治疗手段，并且已经商品化。在免疫系统中，cd147被认为是一个协同刺激分子受体，并能影响胸腺选择的结果。

2021年6月30日 · The knockout of CD147 reduced the invasion and expression of MMP2, MMP7, and MMP9 in cal27 cells: (a) The invasiveness of cells was detected by Transwell assay; (b) Western blotting was used to detect the difference in MMP protein expression between Wild-Type and CD147-Knockout cal27 cells; (c) mRNA expression of proteins Wild-Type and Cas9 ...

2021年9月25日 · Here, we identified CD147 as a universal receptor for SARS-CoV-2 and its variants. Meanwhile, Meplazeumab, a humanized anti-CD147 antibody, could block cellular entry of SARS-CoV-2 and its...