CD14 是一种糖磷脂酰肌醇锚定的质膜蛋白，在 LPS 介导的 TLR4 激活中具有长期确定的作用；CD14 与 LPS 结合并将 LPS 转移到 TLR4-MD2 复合物，从而启动 myddosome 组装和信号转导（Lipopolysaccharide induces alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1α release.

CD14 (cluster of differentiation 14)原称MY23抗原，是一种表面抗原，属细胞表面糖蛋白家族成员之一。 CD14基因编码的蛋白，优先表达于单核/巨噬细胞。 CD14是革兰阴性菌内毒素脂多糖 LPS 和LPS结合蛋白 (LBP)复合物的高亲和力受体。 CD14识别并结合LPS，引起细胞酪氨酸磷酸化、核因子NF-κB转位、触发细胞因子释放和氧自由基产生，在机体免疫、防御系统引起的一系列反应中发挥重要作用。 1. CD14的发现. 1981年，Todd首次从人单核细胞表面发现了CD14。 1985 …

2018年12月1日 · We used computational design methods to predict and optimize a total of 53 cyclic and linear peptides targeting myeloid differentiation 2 (MD2) and cluster of differentiation 14 (CD14), both coreceptors of human TLR4.

2022年12月15日 · We identified CD14 associated with unique characteristics of different serovars as the crucial factor of the TLR4/CD14/MD2 complex for Ct-mediated activation of the NF-κB pathway. Furthermore, we found the TLR4/CD14/MD2 complex to be decisive for the uptake of Ct-derived lipopolysaccharides but not for infection and replication of Ct.

pDUO2-MD2/CD14 is an expression vector designed to co-express MD2 and CD14 genes known to interact with each other : human MD2 (480 bp) and human CD14 (1125 bp) or mouse MD2 (483 bp) and mouse CD14 (1101 bp). The genes cloned into pDUO2 comprise the coding sequence (without introns) from the ATG to the Stop codon.

2017年1月17日 · LPS transfer to TLR4-MD2 is catalyzed by both LPS binding protein (LBP) and CD14. To define the sequential molecular interactions underlying this transfer, we reconstituted in vitro the entire LPS transfer process from LPS micelles to TLR4-MD2.

CD14 first captures and transports LPS to the plasma membrane localized complex of TLR4 and MD2, which signals through the TIRAP-MyD88 adaptors to activate inflammatory cytokine expression. CD14 then transports TLR4 to endosomes by a process mediated by Syk and PLCγ2, where TRAM-TRIF signaling can lead to the expression of IFNs.

2018年6月1日 · Lipopolysaccharide (LPS) from the membrane of Gram-negative bacteria is recognized and extracted by LBP. CD14 then delivers LPS to the TLR4–MD2 complex. Binding of LPS to the TLR4–MD2 complex triggers the dimerization of the cytoplasmic Toll-like interleukin receptor domain (TIR domain) of TLR4 and recruitment of TIR-containing adaptor ...

The lipopolysaccharide (LPS) receptor complex consists of two interacting receptors (CD14 and TLR4) and an associated protein (MD-2). When engaged by LPS, as in gram-negative infection, this complex transduces a signal detected by MyD88 and passed onward by a cascade of the IRAKs, TRAF6, and NIK, re …

2025年3月18日 · The three-dimensional structural pattern diagram of LBP, CD14, TLR4, MD-2, and TLR4-MD-2 complex and the binding pattern diagram of LPS and TLR4-MD2 complex. A LBP comprises two structural domains, each featuring antiparallel β-strands surrounded by an α-helix.