CD11b immunophenotyping identifies inflammatory profiles in …
2015年9月30日 · Overlay of BAL cell (blue) and blood neutrophil (red) staining with anti-CD11b and anti-CD11c Abs shows distinct inflammatory BAL profiles in individual mice (n =3–6, right …
CD11b Marker for Macrophages and Microglia - Bio-Techne
Specific staining was localized to cytoplasm of microglia. Tissue was co-stained with using a Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-Purified Polyclonal Antibody …
Flow cytometric analysis of inflammatory and resident myeloid ...
The myeloid gating strategy allowed natural killer cells (CD11b + NK1.1 +) and neutrophils (CD11b + Ly6G +) to be distinguished from the remaining monocytes and macrophages …
A Protocol for the Comprehensive Flow Cytometric Analysis of …
Neutrophils are distinguished from all other leukocytes (R4) based on their expression of the neutrophil-specific marker, Ly6G. Positive staining for either CD11b or CD11c on non …
Immunohistochemical Identification of Human Skeletal Muscle …
The cell surface localization of CD11b results in better morphological definition of macrophages and more consistent staining across samples than intracellular CD68 staining. Moreover, …
Cleavage of the CD11b extracellular domain by the leukocyte
2011年5月5日 · In this study, we present the first evidence that the shedding of the PMN surface CD11b protein by 3 neutrophil serprocidins (NE, NCG, and NPR-3) plays a critical role in PMN …
Three-colour fluorescence immunohistochemistry reveals the diversity …
2008年5月20日 · Here we have optimised a triple colour immunofluorescent staining protocol, utilising an anti-FITC technique, to allow antibodies to macrophage markers to be used …
CD11b Signaling Prevents Chondrocyte Mineralization and …
2020年12月18日 · Primary murine CD11b KO chondrocytes showed increased mineralization when induced in vitro by secondary calciprotein particles (CPP) and quantified by Alizarin Red …
Temporal pattern of expression and colocalization of microglia ...
2011年12月10日 · Our data show that CD11b staining increases at early time points after ischemia, rapidly reaching a plateau of activation. Notably, CD11b positive cells display a …
Flow cytometric identification and cell-line establishment of ...
2019年11月29日 · CD11b-positive and -negative cells were subdivided by FSC and SSC (Fig. 3b), and sorted cells were observed by Giemsa staining and optical microscopy (Fig. 3c). In …