Molecular Instruments - HCR™ Imaging
The HCR™ Platform is an innovative new method for generalized signal amplification borne out of the fields of synthetic biology and molecular programming. The mechanism is based on two …
Enhanced detection of expanded repeat mRNA foci with hybridization ...
2021年4月23日 · Repeat HCR (R-HCR) provided significantly higher sensitivity for detection of repeats and allowed accurate tracking of endogenous GGGGCC repeat RNAs in patient cells …
HCR™ Amplification Technology | Molecular Instruments, Inc.
HCR™ Amplifiers leverage dynamic nanotechnology to enable small amplification components to first penetrate a biological sample without interacting and then autonomously grow bright …
HCR™ IF + HCR™ RNA-FISHenables a unified approach to multiplexed, quantitative, high-resolution pro-tein immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA …
Optimization and evaluation of fluorescence in situ hybridization …
Using multiple initiator-specific DNA hairpins tagged with different fluorophores (e.g., B1H1-AF647:B1H2-AF647; B2H1-AF488:B2H2-AF488), we were able to detect and directly …
HyPR-seq: Single-cell quantification of chosen RNAs via …
2020年12月12日 · We began by adapting probes from the hybridization chain reaction (HCR) smFISH protocol (24, 25) for a sequencing-based readout. In HCR, two “initiator” probes …
Applications of hybridization chain reaction optical detection ...
2022年1月15日 · Most HCR methods are linear reactions. The cross-opening cascade reaction of two kinetically trapped hairpin probes (H1 and H2) is initiated by one short DNA primer …
课题组工作丨ANGEW丨一种精准可控的DNA编码扩增反应用于活 …
2024年7月12日 · 目前，基于DNA的编码技术和扩增反应，如滚环扩增（RCA）、杂交链反应（HCR）和引物交换反应（PER），为高信号强度、高通量的活细胞膜蛋白成像提供了强有 …
HCR v3.0探针组，放大器和缓冲器-上海起发实验试剂有限公司
2020年9月24日 · HCR v3.0探针集，扩增子和缓冲液可在整个方案中实现多重定量RNA荧光原位杂交（RNA-FISH），RNA流式细胞仪和Northern印迹，并具有自动背景抑制功能。 探针组由分 …
Hybridization chain reaction-based DNA nanomaterials for …
2024年2月1日 · Hybridization chain reaction (HCR) is a simple and efficient isothermal enzyme-free amplification strategy of DNA, generating nicked double helices with repeated units. …