2025年1月21日 · Furthermore, reducing Wapl activity can bypass the need for ATRX to protect centromeric cohesion. These results provide insights into the mechanism of ATRX's centromeric localization and its critical function in preserving centromeric cohesion by …

2022年2月8日 · WAPL deficiency prevents the formation of heterochromatin domains, and allows for gene expression even in the absence of the Mediator-CDK subunit MED12. We propose that cohesin and Mediator...

Taken together, our findings indicate that HP1 ensures appropriate ATRX localization at the mitotic centromere, hence safeguarding centromeric cohesion and preventing chromosomal instability. Additionally, the ATRX-HP1 interaction suppresses Wapl activity.

2020年12月14日 · In this study, we used acute protein degradation to deplete WAPL in mouse embryonic stem cells (mESCs) to examine the immediate effects of changes in cohesin binding and 3D genome changes. We...

2017年6月29日 · At telomeres, TERRA competes with telomeric DNA for ATRX binding, suppresses ATRX localization, and ensures telomeric stability. Depleting TERRA increases telomerase activity and induces telomeric pathologies, including formation of telomere-induced DNA damage foci and loss or duplication of telomeric sequences.

2017年4月19日 · In the absence of both CTCF and Wapl, cohesin accumulates in up to 70 kilobase-long regions at 3′-ends of active genes, in particular if these converge on each other. Changing gene expression...

Inhibiting Wapl activity bypasses the need for HP1 in centromeric cohesion protection. Wapl releases the majority of cohesin from chromosome arms in early mitosis to allow sister‐chromatid resolution 2, 3. Wapl activity in cohesin release is inhibited at mitotic centromeres by Haspin 43, 44, Sororin and Sgo1 59, 62, 63, 64, 65, 66, 67. Given ...

The need for HP1 in cohesion protection can be bypassed by centromeric targeting of Haspin or inhibiting Wapl activity. Taken together, these results reveal a redundant role for HP1α and HP1γ in the protection of centromeric cohesion through promoting Haspin localization at mitotic centromeres in mammalian cells.

We found that plant Like HP1 (LHP1) protein interacts with ATRX through domains that evolved specifically in land plant ancestors. Loss of ATRX function in Arabidopsis affects the expression of a limited subset of genes controlled by PRC2 (POLYCOMB REPRESSIVE COMPLEX 2), including the flowering time regulator FLC. The function of ATRX in ...

2018年8月6日 · HP1 physically interacts with the putative ortholog of the SNF2 chromatin remodeler ATRX, which controls deposition of histone variant H3.3 in mammals. In this study, we show that the Arabidopsis thaliana ortholog of ATRX participates in H3.3 deposition and possesses specific conserved domains in plants.