2021年1月18日 · ATO increased the fluorescence intensity of LC3 and LAMP1, while decreased that of p62 in the aortic sinus, compared to controls (Fig. 7B–E). Furthermore, ATO enhanced LC3 fluorescence...

2024年4月29日 · We observed gradual increases in LC3 lipidation and reductions in p62 protein levels, as well as enrichment of autophagy-associated genes in the ATO-treated H22 cells (Fig. 4G, and...

2024年9月1日 · In a recent study, co-treatment of cervical cancer cells with cyclosporin A and ATO resulted in a significant increase of LC3-II expression compared to single drug treatment. In addition, cyclosporin A also promotes autophagy by inhibiting mTORC1, which could hinder autophagosome formation [16] .

2018年10月19日 · Arsenic trioxide (ATO) is a successful chemotherapeutic drug for blood cancers via selective induction of apoptosis; however its efficacy in solid tumors is limited. Here...

2025年1月17日 · Mechanistically, in vitro studies have shown that nontoxic concentrations of ATO can activate both ERK and phosphoinositide 3-kinase-serine/threonine kinase (PI3K-AKT) pathways and further induce autophagy. The hepatoprotective mechanism of ATO, at least in part, relies on the effects of ATO on the activation of autophagy, which is ERK-dependent.

2012年9月21日 · To investigate whether ATO is a direct activator of autophagic flux, we detected microtubule-associated protein light chain 3 (LC3) (LC3-I/LC3-II) by immunoblot analysis. As shown in Fig.1A, treatment with ATO significantly induced a time-dependent increase in the expression of both LC3-I and LC3-II in K562, Jurkat, MCF-7 and MGC803 cells ...

The autophagy related proteins (LC3 and Beclin1) were detected by immunofluorescence. The cell apoptosis was measured by flow cytometry. Results: With 5-20 μmol/L of ATO, HepG2 cells exposed to 3-MA had significantly lower viability (P <0.05) and higher early apoptosis (P <0.05) than those without exposure to 3-MA. Exposure to 3-MA was also ...

2018年1月29日 · In the present study, we showed that ATO increased autophagic flux in the human osteosarcoma cell line MG-63, as evidenced by the upregulation of LC3-II and downregulation of P62/SQSTM1. Moreover, the pharmacological or genetic blocking autophagy decreased ATO -induced cell death, indicating that ATO triggered autophagic cell …

2023年6月15日 · ATO diminished the number of tumor cells from patients with WT and mutant EGFR, down-regulated the expression or phosphorylation of EGFR, pmTOR, PI3K, PTEN, and p4E-BP1, and up-regulated the expression of LC3. Immunofluorescent experiments showed that ATO enhanced LC3 and P62.

Results With 5- 20 μmol/L of ATO, HepG2 cells exposed to 3-MA had significantly lower viability (P <0.05) and higher early apoptosis (P <0.05) than those without exposure to 3-MA. Exposure to...