在两个类泛素化系统的作用下，细胞质可溶的atg8蛋白连上脂质分子pe，成为膜结合形式的atg8-pe分子，进而定位于自噬体膜。 由于ATG8-PE具有稳定的自噬体定位特征，ATG8-PE一直是自噬领域广为采用的检测自噬体的金标准。

In this review, we comprehensively summarize Atg8-PE protein-based in vitro biochemical approaches and recent advances to facilitate a better understanding of autophagy mechanisms. In addition, we highlight the advantages and disadvantages of various Atg8-PE protein-based approaches to provide general guidance for their use in studying autophagy.

2021年7月8日 · To obtain structural insights into the activity of Atg8–PE to induce membrane area expansion and deformation, Atg8–PE was reconstituted onto lipid bilayer nanodiscs by in vitro lipidation ...

2007年7月13日 · Our results suggest that the membrane-tethering and hemifusion functions of Atg8 are evoked by the conjugation with PE, whereas Atg4 functions as a deconjugase that cleaves the linkage between Atg8 and PE (Kirisako et …

2010年4月16日 · In this study, we developed a method to reliably detect the ATG8-PE adduct and demonstrate its absence in backgrounds containing previously reported or new mutant alleles affecting ATG4a ATG4b, ATG5, ATG7, and ATG10.

2012年5月1日 · Using an Atg8 mutant that bypasses the initial Atg4-dependent processing, we demonstrate that Atg8 deconjugation is an important step required to facilitate multiple events during macroautophagy. The inability to deconjugate Atg8-PE results in the mislocalization of this protein to the vacuolar membrane.

In this review, we comprehensively summarize Atg8–PE protein-based in vitro biochemical approaches and recent advances to facilitate a better understanding of autophagy mechanisms. In addition, we highlight the advantages and disadvantages of various Atg8–PE protein-based approaches to provide general guidance for their use in studying autophagy.

2020年5月5日 · ATG8 lipidation is a hallmark of autophagy where these proteins are involved in autophagosome formation, the fusion of autophagosomes with lysosomes and cargo selection. However, it has become...

Atg8 家族蛋白脂化是监测自噬最常用的标志物。 在巨自噬过程中，Atg8 家族蛋白与磷脂酰乙醇胺 (PE) 特异性结合，形成双膜自噬体。 一种独特的、非经典的自噬途径也起作用，其特征在于 ATG8 与内溶酶体单膜 (CASM) 的结合。

2023年12月6日 · Here we show that Saccharomyces cerevisiae Atg8–PE and E1–E2–E3 enzymes together construct a stable, mobile membrane scaffold. The complete scaffold formation induces an in-bud in prolate-shaped...