2023年6月5日 · We previously found that the knockout (KO) of autophagy-related 5 (Atg5), which is essential for autophagy, leads to the malignant transformation of NIH 3T3 cells. In this study, we explored the mechanism by which autophagy contributes to this malignant transformation using two transformed cell lines, Atg5 KO and Ras-NIH 3T3.

2020年12月4日 · In this study, we investigated the effect of chloroquine (CQ) and its combination with cisplatin (CDDP) in drug-resistant EOC cells. We used the three EOC cell lines CDDP-resistant A2780-CP20,...

Rag-mTORC1激活的一个关键分子是液泡H+ATPase (V-ATPase)，它将ATP水解 (外周V1结构域)与通过溶酶体膜的质子转运体 (完整的V0结构域)结合起来，使溶酶体酸化，使其具有降解功能。 当溶酶体管腔内氨基酸水平较低时，V-ATPase抑制Rag GTP酶的活性。

2012年5月7日 · Our previous work demonstrated that pharmacological inhibition with CQ produces the equivalent antitumor effect as knockdown of an essential autophagy gene ATG5, establishing that the CQ derivatives act as autophagy inhibitors when dosed at low micromolar doses . In this study, we have demonstrated that Lys01 produces more complete lysosomal ...

2012年10月12日 · CQ is only effective in sensitizing starvation-induced cell death in cells with Atg5 expression, but not in Atg5 deficient MEFs and iBMKs. (A and C) Blockage of autophagic flux by CQ in both m5–7 cells and Atg5 Tet-on iBMKs, respectively. Cells were treated with EBSS in the presence or absence of CQ (20 μM) for the indicated period of time ...

2025年1月15日 · We identify lysophosphatidic acid and serine as glycolytic intermediates that trigger PC death and demonstrate that the deletion of GLUT2 in ATG5-deficient mice mitigates PC neurodegeneration and...

Atg5是参与自噬泡中吞噬细胞膜延伸的关键蛋白，它与Atg12形成组成型的复合物。 在此过程中，首先Atg7作为类E1泛素活化酶激活Atg12；然后Atg12被传递给类E2泛素转移酶Atg10；最后Atg12与Atg5结合形成复合物。

2020年7月12日 · Atg5 knockdown in NSFs led to accumulation of dysfunctional mitochondria, and suppressed cell proliferation and cell adhesion ability, promoting apoptosis/anoikis accompanied by upregulation of the apoptosis-related genes, Bax and Noxa. Furthermore, inhibition of the p53/Rb pro-apoptotic pathway with SV40 large T antigen abolished Atg5 ...

通过对哺乳动物的研究揭示，在饥饿信号诱导的自噬先于凋亡的PCD进程中，ATG4、ATG5和Beclin 1的截短型分子ATG4D、ATG5－tN和Beclin 1－C发挥了在细胞自噬过程中诱导细胞凋亡的功能，即ATG4、ATG5和Beclin 1被认为是导致PCD机制中细胞自噬触发凋亡的重要分子开关蛋白 …

2025年3月31日 · 此外，atg5 敲低有效地阻止了 atp6ap1 过表达引起的管腔型乳腺癌细胞增殖的增加。 ... 此外，研究人员利用cq抑制 atp6ap1 过表达的乳腺癌细胞中的自噬，并分析其对肿瘤坏死因子相关凋亡诱导配体（tam）诱导的细胞凋亡的影响。