ATG16L1 protein consists of three main domains - N-terminal region which contains an alpha-helix required for binding to ATG5 ubiquitin -folds, a middle region (coiled-coil domain, CCD), and a domain made of seven WD40 repeats, that forms a β-propeller, found in its C-terminal part.

2018年12月20日 · The evolutionary conserved ATG16 plays a key role in autophagy and appears to link autophagy with the UPS. Here, we review the role of ATG16 in different species. We summarize the current knowledge of its functions in autophagosome membrane expansion and autophagosome formation, in Crohn's disease, and in bacterial sequestration.

Atg 基因通过 Atg12-Atg5 以及 LC3-II (Atg8-II) 复合体，控制自噬体的形成。 Atg12 通过一个需要 Atg7 和 Atg10（分别对应 E1 和 E2 样酶）参与的泛素样反应，结合到 Atg5。 Atg12-Atg5 接合后，与 Atg16 通过非共价相互作用形成一个大复合体。 LC3/Atg8 被 Atg4 蛋白酶在羧基端剪切后产生胞质 LC3-I。 LC3-I 也是通过一个需要 Atg7 和 Atg3（分别对应 E1 和 E2 样酶）参与的泛素样反应，结合到磷脂酰乙醇胺 (PE)。 脂质化形式的 LC3，也称作 LC3-II，附着到自噬体的膜上。 …

与 Atg5-Atg12-Atg16L 不同，LC3B–II 既存在于自噬体的内表面，也存在于自噬体的外表面。自噬膜的延伸和完成离不开 LC3B–II。自噬体膜闭合之后，Atg16-Atg5-Atg12 复合物脱离囊泡，但一部分 LC3-II 仍然与膜共价结合（图 1）。

2018年12月20日 · ATG16 is present in all eukaryotic species and its function is well-studied. ATG16 is required for the correct localization of the ATG12~5 conjugate to the pre-autophagosomal structure in yeast and to the isolation membrane in higher eukaryotes [13,14].

2025年3月28日 · ATG16L1 (Autophagy Related 16 Like 1) is a Protein Coding gene. Diseases associated with ATG16L1 include Inflammatory Bowel Disease 10 and Inflammatory Bowel Disease 1. Among its related pathways are Autophagy and Selective autophagy. Gene Ontology (GO) annotations related to this gene include identical protein binding.

该研究对线虫同源基因atg-16进行深入的遗传和生化分析，阐明了Atg16在多细胞生物中的功能。 张宏课题组利用线虫作为模式生物进行遗传筛选，通过两个不同的筛选模型，得到了线虫的两个atg-16的同源基因：atg-16.1和atg-16.2。

2024年1月4日 · We discovered a neuronal role for C. elegans ATG-16.2 and its WD40 domain in lifespan, proteostasis and exopher biogenesis. Our findings suggest noncanonical functions for select autophagy genes...

2022年10月17日 · Autophagy is a ubiquitous intracellular degradation system for eukaryotic cells. During autophagy, cytoplasmic components are enclosed in autophagosomes and delivered to lysosomes/vacuoles. ATG16 (also known as Apg16) has been shown to be bind to Apg5 and is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway.

2020年5月26日 · Although Atg16/ATG16L1 is dispensable for Atg8/LC3 lipidation reactions in vitro 133,135, Atg16 can enhance Atg8 lipidation activity against low-curvature liposomes 137 and immobilize Atg8-PE...