Therefore, double-label confocal microscopy with markers specific to plaques (β-amyloid), NFTs (AT8), activated astrocytes (glial fibrillary acidic protein [GFAP]) and microglia (CD68) were used to determine the localization of Hic-5 and paxillin in AD brains.

2024年2月6日 · Hyperphosphorylated tau polymerizes into paired helical filaments, which aggregate to form neurofibrillary tangles, one of the defining lesions of Alzheimer’s disease. AT8, which recognizes paired helical filament (PHF) tau, is …

Immunohistochemistry using the AT8 antibody in hippocampal (a,c,e,g) and entorhinal cortex (b,d,f,h) sections from WT (a,b), WT M (c,d), Tg2576 (e,f) and Tg2576 M (g,h) mice. Scale bars: 50 µm....

Immunohistochemistry with the AT8 antibody showing phosphorylated tau (Ser202/Thr205) in the frontal cortex (FC; A,E,I,M,Q), hippocampus (HP; B,F,J,N,R), entorhinal cortex (EC; C,G,K,O,S) and...

2021年5月11日 · Immunofluorescence analysis using Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) (Product # MN1020), shows plasma membrane and cytoskeleton localization in SH-SY5Y cells treated with retinoic acid as compared to untreated SH-SY5Y cells which is reported to be low to negative for PhosphoTau expression.

2016年1月21日 · AT8 is a PHF-tau-specific monoclonal antibody that is a commonly used marker of neuropathology because of its recognition of abnormally phosphorylated tau. Previous reports described the AT8 epitope to include pS202/pT205. Our studies support and extend previous findings by also identifying pS208 as part of the binding epitope.

线性混合效应模型用于测试区域和群体差异，同时根据人口统计数据进行调整。两组患者的海马亚区均表现出 AT8 反应性，这与传统的 Braak 分期相似，在 Braak 分期早期受影响的亚区中磷酸化 tau 蛋白的负担较高。

2007年6月29日 · MAb AT8, which is probably one of the most commonly applied PHF-tau specific mAbs to stain NFTs histochemically, was shown to recognize tau phosphorylated around positions 199–205 [18], [19], [20]. However, different phosphorylation degrees were reported for the epitope using protein mutation studies.

Comparison of (A) MC-1 and (B) AT8 immunohistochemical (IHC) scores with relative insoluble tau amount as determined by ELISA analysis of brain homogenates for each vehicle-and EpoD-treated PS19...

AT8染色是一种常用的免疫组化染色方法，通过特异性抗体识别和染色异常磷酸化的Tau蛋白，从而帮助研究人员观察和评估神经退行性疾病的发生和发展。