Ara h 3 is a seed storage protein from Arachis hypogaea (peanuts). [1] It is a heat stable 11S legumin-like globulin [2] with a stable trimeric form that comprises 19% of the total protein in peanut extracts.

2009年5月1日 · Here we report the crystal structure of Ara h 3 at 1.73 Å resolution. Mapping of the previously defined linear epitopes on the crystal structure of Ara h 3 indicated that linear epitopes with more solvent exposure were those indicated …

An immunodominant epitope on Ara h 3 was characterized. Click on the arrows to show the details of individual isoallergens, click on +/- to show/hide the details of all isoallergens.

IgE-binding properties of Ara h 3 were investigated using direct elisa and Western blotting with sera from peanut-allergic individuals. The basic subunits, and to a lesser extent the acidic subunits, bind IgE and may act as allergenic peptides.

2024年7月1日 · A sandwich ELISA was developed to quantify peanut allergen Ara h 3 in foods. Ara h 3 was purified by salt precipitation following with gel filtration chromatography. After animal immunization, anti-Ara h3 antibody was produced as capture and detection antibody, to develop sandwich ELISA.

2023年5月1日 · To elucidate the vital function of roasted peanut allergen Ara h 3 in anaphylaxis, the changes of structure and physicochemical properties of allergen Ara h 3 after roasting were studied. The result proved that Ara h 3 proteins were recognized and uptake by …

RATIONALE: Ara h 3 is a major peanut allergen. Linear IgE-binding epitopes in this allergen have been defined before. Determination of the three dimensional structure of Ara h 3 and mapping of the linear epitopes are required to understand the relationship between structure and allergenicity and to develop better treatment.

Major peanut allergens Ara h1, Ara h2 and Ara h3 were purified from peanuts using ion-exchange chromatography. IgE immunoblotting was performed and IgE-cross-linking capacity was examined by measuring histamine release (HR) after incubating patient basophils as well as passively sensitized basophils with several dilutions of the allergens.

2003年10月17日 · Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its ...

The allergen Ara h 3 has been purified recently from peanuts. In contrast to recombinant Ara h 3, a 60 kDa single-chain polypeptide, the allergen isolated from its native source is extensively proteolytically processed. The characteristic proteolytic …