beta-Galactosidase alpha-complementation. Overlapping …
Treatment of M112 protein/3-41 peptide complemented enzyme with trypsin under very mild conditions followed by examination of the products demonstrated that the alpha-donor pep]tide supplies the NH2-terminal segment of complemented enzyme.
beta-Galactosidase alpha-complementation. Overlapping …
1981年7月10日 · An important advance in understanding the molecular nature of a-complementation was made when it was shown that complementation can occur between M15 protein and CNBr2, a cyanogen bromide peptide derived …
Molecular basis of beta-galactosidase alpha-complementation.
We have now isolated the defective beta-galactosidase alpha-acceptor protein from the deletion mutant strain M15 of Escherichia coli and find that it lacks residues 11-41 of betal-galactosidase. This is demonstrated by the isolation and sequence determination of a cyanogen bromide peptide from the M15 protein, which is identical to the ...
beta-Galactosidase alpha-complementation. A model of …
1982年11月26日 · Studies on beta-galactosidase alpha-complementation are reviewed. The isolation and structure of two beta-galactosidase fragments that form an enzymically active complex are described. One of these is a cyanogen bromide peptide from whole beta-galactosidase; the other is a dimeric protein from a lac …
β-Galactosidase α-complementation - Molecular and Cellular …
Studies on β-galactosidase α-complementation are reviewed. The isolation and structure of two β-galactosidase fragments that form an enzymically active complex are described. One of these is a cyanogen bromide peptide from whole β-galactosidase; the other is a dimeric-protein from a lacZ deletion mutant of Escherichia coli.
Molecular basis of beta-galactosidase alpha-complementation
In previous studies, a cyanogen bromide peptide derived from amino-acid residues 3-92 of beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) was shown to have alpha-donor activity in intracistronic alpha-complementation. We have now isolated the defective beta-galactosidase alpha-a …
High resolution refinement of beta-galactosidase in a new crystal …
High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation. The unrefined fold of Escherichia coli beta-galactosidase based on a monoclinic crystal form with four independent tetramers has been reported previously.
beta-Galactosidase alpha-complementation. Overlapping sequences
1981年7月10日 · Cleavage of CNBr2 with glutamic acid-specific protease yielded a much smaller alp ha-donor (3-41 peptide) which was also effective in complementation, indicating that the M15 protein can supply all of the residues from 42-92 for the structure of complemented enzyme.
Beta-galactosidase Alpha-Complementation - Molecular Depot
2019年4月14日 · Beta-galactosidase Alpha-Complementation is a biochemical phenomenon first documented by Agnes Ullmann, while working in the lab of François Jacob and Jacques Monod. By means of molecular cloning, the native E. coli β-galactosidase enzyme can be split in two inactive fragments of different sizes.
beta-Galactosidase alpha complementation: properties of the ...
Intracistronic alpha complementation involving Escherichia coli beta-galactosidase occurs between the cyanogen bromide peptide CB2, derived from residues 3-92 of beta-galactosidase (Langley, K.E., Fowler, A.V., and Zabin, I. (1975), J. Biol. Chem. 250, 2587), and the defective beta-galactosidase from the Z-deletion mutant strain M15.