2023年4月12日 · 为了重现在体内观察到的伪分层粘膜纤毛 表型（pseudostratified mucociliary phenotype），必须在气-液界面（AirLiquid Interface，ALI）上培养原代人支气管上皮细胞（primary human bronchial epithelial cells，HBEC）（图1） 6 。气-液界面培养的主要特征是细胞的基底表面与液体培养基 ...

2023年3月1日 · IL-13 induces mucus-producing cells, metaplasia, or hyperplasia and increases the expression and production of MUC5AC, which are the main characteristics of chronic bronchitis. Hence, treating HBE cells with IL-13 under ALI conditions promotes the development of a chronic bronchitis-like mucosa [85].

2023年6月22日 · Here we report the development of a planar airway 3D organ tissue equivalent (OTE) model, comprised of a well-differentiated HBE layer at ALI, maintained on a hydrogel substrate layer that can...

2024年10月15日 · Multiplex single-cell RNA sequencing of ALI-HBE was used to reveal previously unrecognized transcriptional heterogeneity within the human bronchial epithelium and cell type–specific responses to acute exposure to e-cigarette aerosol (e-aerosol) containing distinct components (nicotine and/or flavoring).

2019年1月24日 · NHBE cells expanded in M3 expansion medium to passage 1, 2, 4, 6 and 8 were cultured at ALI for 4 weeks in ALI differentiation media. The resulting 3D cultures were stained with H&E.

In order to recapitulate the pseudostratified mucociliary phenotype observed in vivo, primary human bronchial epithelial cells (HBECs) must be cultured at the air-liquid interface (ALI; Figure 1). 6 The defining feature of ALI culture is that the basal surface of the cells is in contact with liquid culture medium, whereas the apical surface is ...

In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression ...

2010年8月19日 · Human bronchial epithelial (HBE) cells grown on collagen-coated Transwell membranes under air–liquid interface (ALI) conditions differentiate to form an epithelium with ciliated, goblet, and basal cells that morphologically mimics human airway epithelium in vivo [reviewed in (18, 19)].

2021年5月26日 · Here, we present the efficacy and utility of a simple method for siRNA transfection of HBEs in ALI cultures that does not require potentially cytotoxic transfection reagents and does not detrimentally alter the physiology of …

Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method. NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/- …