The overall reaction catalysed by the aldehyde dehydrogenases is: RCHO + NAD + + H 2 O → RCOOH + NADH + H + In this NAD (P) + -dependent reaction, the aldehyde enters the active site through a channel extending from the surface of the enzyme.

Aldehyde dehydrogenases (ALDH) (EC 1.2.1.3) are a family of nicotinamide adenine dinucleotide (phosphate) (NAD (P)) dependent enzymes, typically with a molecular mass of ca. 50–60 kDa. They oxidise a large range of aliphatic and aromatic, endogenous and exogenous aldehydes to form the corresponding carboxylic acids.

The main pathway of ethanol metabolism involves its conversion (i.e., oxidation) to acetaldehyde, a reaction that is mediated (i.e., catalyzed) by enzymes known as alcohol dehydrogenases (ADHs). In a second reaction catalyzed by aldehyde dehydrogenase (ALDH) enzymes, acetaldehyde is oxidized to acetate.

As a direct consequence of their significant physiological and toxicological roles, inhibitors of the ALDH enzymes have been developed to treat human diseases. This review summarizes known ALDH inhibitors, their mechanisms of action, isozyme selectivity, potency, and clinical uses.

2019年10月4日 · Aldehyde-alcohol dehydrogenase (AdhE) is a key enzyme in bacterial fermentation, converting acetyl-CoA to ethanol, via two consecutive catalytic reactions. Here, we present a 3.5 Å resolution...

2021年5月14日 · An in-depth understanding of the ALDH structure-function relationship and mechanism of action is key to the understanding of associated diseases. Principal structural features 1) cofactor binding domain, 2) active site and 3) oligomerization mechanism proved critical in maintaining ALDH normal activity.

Aldehyde dehydrogenases (ALDHs), broadly defined as a superfamily of NADP (+)-dependent enzymes, participate in aldehyde metabolism, catalysing the oxidation of exogenous aldehydes (drugs and ethanol) and endogenous aldehydes (lipid, amino acids, or vitamins) into their corresponding carboxylic acids [1].

The catalytic reaction occurs in five steps: first Cys-302 is activated by a water-mediated proton abstraction by Glu-268, then the thiolate group of Cys-302 attacks the electrophilic aldehyde which forms a tetrahedral thiohemiacetal intermediate and a hydride transfer to the NAD + ring, resulting in a thioester intermediate which is hydrolyzed ...

2018年2月13日 · Aldehyde dehydrogenase enzymes (ALDHs) catalyze the oxidation of aliphatic and aromatic aldehydes to their corresponding carboxylic acids using NAD + or NADP + as...

2021年5月13日 · Aldehyde dehydrogenases (ALDH) (EC 1.2.1.3) are a family of nicotinamide adenine dinucleotide (phosphate) (NAD (P)) dependent enzymes, typically with a molecular mass of ca. 50–60 kDa. They oxidise a large range of aliphatic and aromatic, endogenous and exogenous aldehydes to form the corresponding carboxylic acids.