Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt (ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination.

常见的有 TdTomato鼠 、 mT/mG鼠 、 LacZ鼠 、彩虹鼠 等，他们的重要作用是对各种特异性表达的Cre工具鼠进行验证，简而言之就是“工具鼠的工具鼠”：前面我们关于 Cre-loxp系统 的介绍中大家已经知道了带有特异性表达的Cre工具鼠，然而如何去验证这种鼠的特异性表达呢？ 前人们巧妙地设计了 reporter鼠来指示Cre鼠的特异性。 B-tdTomato cKI大鼠. TdTomato大鼠（SD- Gt (ROSA26)tm1 (CAG-LSL-Tdtomato) /Bcgen）在ROSA位点敲入了一个CAG启动子 …

When intravenously administered into mice, only rAAV9 was able to transduce osteoblasts and osteoclasts on the bone surface and osteocytes embedded within the bone matrix. As previously reported,...

Ai9小鼠是一种经过基因修饰的小鼠品系，常用于荧光示踪。 Ai9小鼠携带一个被loxP位点夹住的STOP盒子，这个STOP盒子阻止了tdTomato红色荧光蛋白的转录。 当Ai9小鼠与表达Cre重组 …

Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt (ROSA)26Sor locus.

Comparing recombination in mice with the Ai9 versus the R26-YFP recombination reporter, we observed significantly fewer recombined IBA1 + brain macrophages in Tmem119CreER and P2ry12CreER lines with R26-YFP (94.55% for the Tmem119CreERAi9 mice and 95.81% for the P2ry12CreERAi9 mice vs. 33.78% for Tmem119CreERR26-YFP and 36.51% for P2ry12CreERAi9...

2020年6月26日 · Here, we report a generalizable methodology that allows engineering of modified lipid nanoparticles to efficiently deliver RNPs into cells and edit tissues including muscle, brain, liver, and...

2009年12月20日 · This Resource paper describes a set of five new reporter mice, derived from Rosa26, driving Cre-dependent strong and ubiquitous expression of fluorescent proteins.

2024年10月28日 · Ai6-HDR1 and Ai6-HDR2 offer detection of homology-directed repair events by native fluorescence (RRIDs MMRRC_071742-JAX, MMRRC_071743-JAX). Ai9-SauSpyCas9 replicates the original Ai9, but with identical sequences flanking the stop cassette, allowing the use of a single guide RNA for CRISPR/Cas-driven activation (RRID MMRRC_068227-JAX).

2024年6月1日 · The Ai9 were crossed with the Sox2-Cre line to generate the target tdTomato mice expressing tdTomato in epiblast derived tissues (Hayashi et al., 2002b; Madisen et al., 2010b) (Fig. 1 A, bottom). The retina is also developmentally originated from epiblast ( …