ADP-Glo™ Kinase Assay (ADP-Glo™ 激酶检测试剂盒) 是一个发光法激酶检测试剂盒，它检测激酶反应中所形成的ADP；ADP被转化成ATP，然后ATP 再被Ultra-Glo™ 萤光素酶转化成光。

The ADP-Glo™ Kinase assay has a high dynamic range and produces a strong signal at low ATP-to-ADP conversion, making it well suited for screening low-activity kinases such as growth factor receptor tyrosine kinases. The assay can be used at ATP concentrations up to 1mM, important for kinases with high K m values for ATP.

ADP-Glo™ Kinase Assay (a-f) 是一款生物发光法的ADP 检测试剂盒。本试剂盒提供了一种通用、均质、高通量筛选方法， 可用于定量激酶反应过程中ADP 生成量，从而检测激酶活性。ADP-Glo™ Kinase Assay可用于监测几乎所有ADP 生

ADP-Glo™ (原理见图1)是Promega公司开发的一种发光法激酶检测方式，检测激酶反应中所形成的ADP的量来反应激酶活性。 在该检测方法中，激酶反应中剩余的ATP会首先被ADP-Glo试剂消耗掉；接下来，激酶反应中生成的ADP则会被激酶检测试剂还原成ATP；然后，ATP在Ultra-Glo™萤光素酶的作用下，与荧光素反应发光，发光信号与激酶活性正相关。 ADP-Glo™可用于检测几乎任何可生成ADP的酶的活性，不需要抗体参与以及放射性标记。 这种检测方法相当敏感，能够 …

The ADP-Glo™ Kinase Assay(a–e) is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure kinase activity by quantifying the amount of ADP produced during a kinase

The ADP-Glo™ Kinase Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure kinase activity by quantifying the amount of ADP produced during a kinase reaction.

ADP-Glo™ 脂激酶检测系统提供了一种便捷灵敏的方法来测量所有类型的磷酸肌醇脂激酶(PIKs) 活性。 该方法可在96 孔板或384 孔板中进行，可用于酶学表征、抑制剂筛选或化合物分析。

The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.

The ADP-Glo™ Kinase Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure kinase activity by quantifying the amount of ADP produced during a kinase reaction.

The ADP-Glo™ Kinase assay has a high dynamic range and produces a strong signal at low ATP-to-ADP conversion, making it well suited for screening low-activity kinases such as growth factor receptor tyrosine kinases. The assay can be used at ATP concentrations up to 1mM, important for kinases with high K m values for ATP.