2020年5月12日 · In this study, cellular carbon fluxes in the acetoin producer CGR6 were further redirected toward acetoin synthesis using several metabolic engineering strategies, including blocking anaplerotic pathways, attenuating key genes of the TCA cycle and integrating additional copies of the alsSD operon into the genome.

2024年2月21日 · Incorporating the PHB synthesis pathway into Bacillus subtilis 168 has been shown to significantly enhance the strain’s acetoin tolerance. This study aims to elucidate the molecular mechanisms underlying the response of B. subtilis 168-phaCBA to acetoin stress, employing transcriptomic and metabolomic analyses.

2014年3月1日 · Acetoin can be synthesized in mammals including human and a pathway with acetoin as a key metabolite was proposed to explain the detoxication mechanism of acetaldehyde (Otsuka et al., 1996, Otsuka et al., 1999). Acetoin can also be produced in plant cells, such as tobacco, carrot, maize and rice (Forlani et al., 1999).

Acetoin (3-hydroxy-2-butanone) is an important physiological metabolic product in many microorganisms. Acetoin breakdown is catalyzed by the acetoin dehydrogenase enzyme system (AoDH ES), which is encoded by acoABCL operon. In this study, we analyzed transcription and regulation of the aco operon in …

Acetoin biosynthesis pathway in L. lactis MG1363. Als, acetolactate synthase; Ald, acetolactate decarboxylase. Acetoin contains a hydroxyl (R-OH) and a keto (R 1 [C═O]R 2) group. Lactococcus lactis species, widely used in the food industry, produce mainly lactic acid from carbohydrates via a fermentative metabolism.

2015年3月1日 · In the present study, we attempted to introduce a heterologous acetoin pathway in the mitochondrion of Candida glabrata and achieved high acetoin accumulation. As a member of the C4-compound family, acetoin has been defined as one of the potential top 30 sugar-derived chemical building blocks by the U.S. Department of Energy (Werpy et al., 2004).

In this review, we first examine the acetoin synthesis pathways and its physiological meanings and relevancies; then we discuss the relationship between the two conflicting acetoin cleavage pathways, the enzymes of the acetoin dehydrogenase enzyme system, major genes involved in acetoin degradation, and the CcpA mediated acetoin catabolite ...

2008年10月11日 · In this review, we first examine the acetoin synthesis pathways and its physiological meanings and relevancies; then we discuss the relationship between the two conflicting acetoin cleavage pathways, the enzymes of the acetoin dehydrogenase enzyme system, major genes involved in acetoin degradation, and the CcpA mediated acetoin catabolite ...

2020年5月1日 · Acetoin (3-hydroxy-2-butanone) is an important physiological metabolic product in many microorganisms. Acetoin breakdown is catalyzed by the acetoin dehydrogenase enzyme system (AoDH ES), which is encoded by acoABCL operon. In this study, we analyzed transcription and regulation of the aco operon in Bacillus thuringiensis (Bt).

2018年8月31日 · Our results showed that two metabolic pathways (acetate and acetoin) are key elements of respiration. The acetate pathway contributes to biomass yield. The acetoin pathway is needed for pH homeostasis, which affects metabolic …