2020年9月24日 · ATR responds to mechanical stress at the nuclear envelope and mediates envelope-associated repair of aberrant topological DNA states. By combining microscopy, electron microscopic analysis,...

2023年3月1日 · To develop an ATM reporter for imaging endogenous ATM activity in living cells, we engineered GFP so that upon phosphorylation by ATM, it undergoes phase separation forming intensely bright droplets. In particular, we designed a multivalent interaction system to drive ATM activity–dependent phase separation of GFP.

2017年12月14日 · Here, we investigate roles of nuclear PPIs in DNA damage response by sequestering specific PPIs with the expression of nuclear-targeted PH domains, which inhibits recruitment of Ataxia...

2020年9月1日 · Histone H2B-GFP and mCherry-α-tubulin HeLa cells were treated with 20 μM XL413 for 16 h. ATR was inhibited by addition of 5 μM AZD673 and live cell imaging was performed for 4 h. The video shows the progression of three cells that display lagging chromosomes and undergo mitotic catastrophe.

2023年10月19日 · The ataxia telangiectasia mutated and Rad3-related (ATR) kinase is a master regulator of cellular responses to DNA damage and DNA replication stress in human cells. 1 In response to DNA damage or replication stress, the ATR-ATRIP kinase complex is activated following recruitment to DNA damage sites or stalled replication forks. 2 During S phase ...

2005年12月4日 · Here, we show that ATM and the nuclease activity of meiotic recombination 11 (Mre11) are required for the processing of DNA double-strand breaks (DSBs) to generate the replication protein A...

2013年8月8日 · Many of the key proteins initiating the checkpoint signal have been identified and characterised in yeast. Here we explore the role of the ATR activating domain (AAD) of TopBP1 in embryonic development, cell growth and checkpoint activation using a mouse model.

2024年8月1日 · New ATR target SCAF1 interacts with phospho-CTD of RNA Pol II and is recruited to DNA damage sites. SCAF1 depletion partially restores PARP inhibitor resistance and HR in BRCA1-deficient cells. The ATR kinase protects cells against DNA damage and replication stress and represents a promising anti-cancer drug target.

As a general readout for analysis of ATR abundance, subcellular localization and function we employed the reporter U2OS cells expressing GFP-labeled ATR (U2OS ATR-GFP) that allowed us to directly assess also recruitment of the ATR protein to acutely inflicted DNA lesions induced by laser microirradiation of psoralen pre-sensitized cell nuclei ...

2001年11月23日 · We report the identification of an ATR-interacting protein (ATRIP) that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. ATR and ATRIP both localize to intranuclear foci after DNA damage or inhibition of replication.