The altered Schaedler flora (ASF) is a community of eight bacterial species: two lactobacilli, one Bacteroides, one spiral bacterium of the Flexistipes genus, and four extremely oxygen sensitive (EOS) fusiform-shaped species.

2019年1月1日 · Herein, we describe the use of the altered Schaedler flora (ASF) mouse model as an alternative to conventionally-reared and germ-free animals. Colonized with only 8 bacterial species, use of ASF mice greatly simplifies the examination of microbiota-host interactions.

2022年10月26日 · The altered Schaedler flora (ASF) consortium is a well-established defined microbiota model, where mice are stably colonized with 8 distinct murine bacterial species. To take better advantage of the ASF, we established new experimental and bioinformatics resources for researchers to make better use of this model as an experimental system to ...

Animals colonized with a defined microbiota represent useful experimental systems to investigate microbiome function. The altered Schaedler flora (ASF) represents a consortium of eight murine bacterial species that have been used for more than 4 decades where the study of mice with a reduced microbiota is desired.

2016年11月8日 · We found that Parabacteroides ASF519 is a major contributor to the unique qualities of the ASF, with impressive coverage of genes and metabolic activities that may be vital in the wild mouse ...

In contrast to germ-free mice, or mice colonized with only one or two species, ASF mice show the nor-mal gut structure and immune system development. To further expand the utility of the ASF, we have developed technical and bioinformatic resources to enable a systems-based analysis of microbiome function using this model.

2014年4月10日 · The altered Schaedler flora (ASF) is a bacterial community that supports normal growth and development of gnotobiotic mice. We report here the draft genome sequences of the 8 bacteria that comprise the ASF.

2022年8月15日 · ASF with predicted functions encoded within the microbiota of CONV mice (Data Set S1 and S2). This resource can be used in a reductionistic approach to interrogate the

ASF-colonized mice are monitored for contamination by culture and examination of fecal Gram stains and wet mounts as described for germfree mice, taking into account the organisms that should be present. Each ASF organism has characteristic morphology, best appreciated by use of phase-contrast microscopy (Orcutt et al., 1987).

Animals colonized with a defined microbiota represent useful experimental systems to investigate microbiome function. The altered Schaedler flora (ASF) represents a consortium of eight murine bacterial species that have been used for more than 4 decades where the study of mice with a reduced microbiota is desired.