2010年6月11日 · ANT1-silencing strongly reduces ADF and U87-MG cell viability. We evaluated the effect of ANT specific isoform down-regulation on ADF cell viability by crystal violet assay. ANT2 down-regulation by ANT2 siRNA transfection did not alter cell viability.

2017年2月16日 · Mice have three paralogous ANT genes, Ant1, Ant2 and Ant4 (ref. 5). Ant1 is highly expressed in skeletal and cardiac muscle, and its disruption leads to exercise intolerance and cardiomyopathy...

2021年11月9日 · We found that EBV‐LMP1 can localize to the mitochondria to bind directly to adenine nucleotide translocase‐1 (ANT1), fixing the ANT1 conformation in the m‐state, thereby increasing the mitochondrial membrane potential and promoting the viability of NPC cells.

2012年11月22日 · Investigation of fluorescence of JC-1 in solution and in cells using spectrofluorimetry, microscopy and flow cytometry reveals that excitation at 405 nm wavelength, now available on standard...

2023年10月27日 · Adenine nucleotide translocase-1 (ANT1) is expressed in the heart–muscle–brain and has been shown to play a role in a variety of myocardial diseases. Heger et al. demonstrated that transgenic overexpression of the ANT1 protects cardiomyocytes against TGFβ 1 -induced apoptosis by stabilization of the mitochondrial permeability transition ...

2009年7月31日 · In this study we provide in vitro and in vivo evidence showing that the protein disulphide isomerase (PDI) activity of type 2 transglutaminase (TG2) regulates the correct assembly and function of...

2025年2月26日 · Adenine nucleotide translocase 1 (ANT1), involved in exchanging ATP and ADP across the mitochondrial inner membrane, is downregulated in mouse brains with Parkinsonian variations.

Using the mitochondrial potential (ΔΨ m) marker JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) and high-resolution imaging, we functionally analyzed mitochondria in cultured rat hippocampal astrocytes. Ratiometric detection of JC-1 fluorescence identified mitochondria with high and low ΔΨ m.

Experimental results reveal that ANT1 does not contribute to a basal proton leak. Instead, it mediates H+ transport only in the presence of long-chain fatty acids (FA), as already known for UCPs.

JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ).