2022年5月30日 · We set out to investigate whether BAP1 functions in the repair of UV-induced DNA damage. BAP1 depletion rendered 293T cells hypersensitive to UV-C light (254 nm, hereafter denoted UV) (Fig....

2022年5月31日 · Making full use of lentiviral vector technology and the structure of the exosomal membrane, we developed an engineered HEK-293T exosome-based delivery system that intelligently integrates three functions: tumor targeting, 131 I and Dox coloading with high payloads, and tumor-targeted therapy.

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.

2021年11月1日 · For quantitative analysis of UV-induced RPA2 focus formation, the 293T or POLH-1 cells transfected with corresponding plasmids were treated with UV. Images were taken with the same exposure...

2011年3月18日 · We report that PIDD modulates p21-PCNA dissociation, and promotes PCNA monoubiquitination and interaction with pol η in response to UV irradiation. Furthermore, PIDD deficiency leads to a defect...

293T细胞能够复制携带SV40复制区的载体，当用于生产 逆转录病毒 时，会产生高滴度病毒。 293T细胞已广泛用于逆转录病毒生产、基因表达和蛋白质生产。 细胞贴壁比较弱，因此在遇到运输低温及震荡、室温静置太长时间、添加的培养基或其他试剂过冷、密度较高、聚集未吹散、加液吹打到细胞面等情况时会出现明显的成片脱落现象，此时若脱落现象不严重应尽快放回培养基继续培养，若呈大片脱落的情况时需要收集细胞重新消化吹散并接种； 建议使用经过包被或者高 …

1， 胰酶消化过度：293T的消化条件一般为胰酶（0.25%）1mL，37℃培养箱处理45s-1.5min； 问：要是不小心消化了15min怎么办？ 答：其实问题不大，比如你消化后要1:5传，现在换成1:4传代就好，放24h差不多就好了。

HEK-293T人胚肾细胞：人胚肾细胞293细胞插入SV40 T-抗原基因后产生的高转染效率的衍生株称为293T。 该细胞最初的名字是293tsA1609neo，携带SV40复制序列，被广泛用于逆转录病毒生产、基因表达和蛋白表达。 HEK-293FT人胚肾细胞：该细胞稳定表达SV40大T抗原，并且促进最适病毒产物的产生。 293FT细胞能制造高滴度的慢病毒。 293A人胚肾细胞:细胞来源于人肾纤维母细胞，组成性表达 E1A 和 E1B 蛋白。 从背景知识我们应该知道怎么选择所需求的细胞了吧! 形 …

Reagents and equipment for ligand binding, UV-cross-linking, and sample preparation • HEPES dissociation buffer (HDB): 12.5 mM HEPES, 140 mM NaCl, 5 mM KCl • Ligand-binding buffer: HDB supplemented with 5 mM MgCl 2, 0.1% (w/v) BSA, and 0.01% (v/v) Triton X-100 •

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.