PXR Functionally Interacts with NF-κB and AP-1 to Downregulate …
Effect of PXR on NF-κB- and AP-1-mediated gene transcription. 293T cells were transfected with NFκB-Luc (A) or AP1-Luc (B), mPXR and/or GRIP1 expression plasmid and/or their control …
Acetylation of Pregnane X Receptor protein determines selective ...
To investigate whether PXR is acetylated, we transfected 293T cells either with a pcDNA-His-hPXR expression construct or with only the pcDNA-His vector plasmid (control) and subjected …
The F-box-only protein 44 regulates pregnane X receptor protein …
2023年11月1日 · The FBA domain of FBXO44 interacts with the LBD of PXR. (A) 293T cells were transfected with the indicated constructs for 48 h before lysis and immunoprecipitation with …
Acetylation of lysine 109 modulates pregnane X receptor DNA …
2016年9月1日 · Given that p300 acetylates PXR, we tested the functional effect of p300 on PXR transcriptional activity in cell-based gene reporter assays performed in 293T cells. Co …
Stability of the human pregnane X receptor is regulated by E3 …
2014年4月1日 · HEK 293T cells stably expressing FLAG-hPXR (fPXR) were used to establish stable knockdown of UBR5, DYRK2, or non-targeting control. Briefly, HEK 293T/fPXR cells …
PXR Functionally Interacts with NF-κB and AP-1 to ... - PubMed
2020年10月15日 · Reporter assays with wild-type and mutated mouse Cxcl2 promoter-containing reporter plasmids were conducted in 293T cells. Results showed that the hepatic expression …
PXR在功能上与NF-κB和AP-1相互作用，以下调炎症诱导的趋化因 …
在293T细胞中使用野生型和突变的含小鼠Cxcl2启动子的报告基因进行报告基因分析。 结果表明，炎症相关基因的肝表达在CCl中上调用4处理的小鼠和PCN处理抑制了所研究基因中编码趋 …
孕烷X受体双荧光素酶报告基因方法建立及有机磷酸酯阻燃剂的受 …
In this study, the pBIND-hPXR expression plasmid containing a renilla luciferase reporter gene was constructed based on the recombinant DNA technology, and then co-transfected with a …
293、293T、293FT 、293A的区别和培养攻略 - BIOON
HEK-293T人胚肾细胞：人胚肾细胞293细胞插入SV40 T-抗原基因后产生的高转染效率的衍生株称为293T。 该细胞最初的名字是293tsA1609neo，携带SV40复制序列，被广泛用于逆转录病毒 …
Structure-guided approach to modulate small molecule binding to …
Structural predictions and biochemical results translate to cellular PXR activity. 293T cells were cotransfected with a PXR-responsive luciferase reporter and either an empty vector (EV) or …