MiR-23a-3p was compared between FECD model and normal control, and the target gene PTEN was confirmed through Western blot and dual-luciferase reporter assays. Treatment with PTEN, PI3K, Akt, and mTOR inhibitors provided insights into the role of the PTEN/PI3K/Akt/mTOR pathway in FECD model.

2011年8月9日 · Results: miR-23a expression was significantly downregulated in macular RPE cells from AMD eyes. H(2)O(2)-induced ARPE-19 cell death and apoptosis were increased by an miR-23a inhibitor and decreased by an miR-23a mimic.

2025年1月1日 · MiR-23a-3p mitigates ferroptosis by downregulating PTEN in CEnCs. Fuchs endothelial corneal dystrophy (FECD) is the leading cause of keratoplasty without drug treatment. Research indicated that oxidative stress and lipid peroxidation play significant roles in FECD.

2021年2月26日 · In the present study, miR-23a-3p was predicted and verified to target BCL2, an anti-apoptotic gene family member, in HLE-B3 cells, which may improve the current understanding of the role of miR-23a-3p in numerous types of human disease (24,25).

2021年10月25日 · In the present study, results from bioinformatics prediction indicated that miRNA-23a was an upstream miRNA regulating VEGF, with similar and highly conservative sequences within the targeted regions in human and rat models. miRNA-23a is widely involved in the regulation of various physiological and pathological processes in the body, which ...

2024年8月5日 · Our study showed that melatonin could ameliorate H 2 O 2-induced apoptosis and oxidative stress injury in RGC cells by mediating miR-23a-3p/Nrf2 signaling pathway, thereby improving retinal degeneration.

2024年7月5日 · Circ_0122396 may regulate the progression of ARC via the miR-23a-3p/MMP16 pathway in H 2 O 2-stimulated HLECs, which may serve as a potentially valuable biomarker and novel therapeutic target for ARC.

Numerous microRNAs (miRs) have been reported to regulate apoptosis in LECs. For instance, miR-23a expression levels were shown to be upregulated in cataractous lenses; however, the function of miR-23a in cataracts remains undetermined. To establish an in vitro model of cataracts, human LECs, HLE-B3 cells, were induced with 200 µmol/l H 2 O 2 ...

MiR-23a-3p was compared between FECD model and normal control, and the target gene PTEN was confirmed through Western blot and dual-luciferase reporter assays. Treatment with PTEN, PI3K, Akt, and mTOR inhibitors provided insights into the role of the PTEN/PI3K/Akt/mTOR pathway in FECD model.

2013年10月10日 · We generated a murine model overexpressing microRNA-23a (miR-23a) to investigate its role in myelin regulation. In addition, we used this mouse model to identify two targets of miR-23a: one is a protein-coding gene phosphatase and tensin homologue and the other is a long ncRNA (lncRNA) 2700046G09Rik.