
Pathology Outlines - Mycobacteria non-TB
2022年8月30日 · Acid fast special stains including the classic Ziehl-Neelsen (ZN) stain and modified Kinyoun stain, also known as cold Ziehl-Neelsen technique Both stain mycobacteria as pink-red slender rods Auramine-rhodmaine fluorescent stain is more sensitive than acid fast stain under light microscopy ( Wilmott: Kendig's Disorders of the Respiratory Tract ...
Pathology Outlines - Actinomyces
2022年8月16日 · C. Modified Kinyoun. Actinomyces species, unlike Nocardia species, do not have high levels of mycolic acid in the cell wall and will not retain the carbol fuschin dye when using a weaker acid for decolorization (i.e., modified acid fast stain), resulting in a negative stain. Nocardia will retain
Pathology Outlines - Cyclospora cayetanensis
2019年8月8日 · Oocyst in stool with modified acid fast stain Diagnosis can be made by the shape and size of oocysts (~8 micrometers in diameter) (Pritt: Creepy Dreadful Wonderful Parasites Blog - Answer to Case 551 [Accessed 8 August 2019])
Pathology Outlines - Tuberculosis
2023年10月9日 · Bacteria stain bright red by the Ziehl-Neelsen / Kinyoun method Fluorochrome method is used by large laboratories that have a fluorescent (ultraviolet) microscope Finding a small acid fast bacillus in a ZN stained slide is a cumbersome procedure for a pathologist; therefore, an artificial intelligence based method can be used to detect ...
Pathology Outlines - Reticulin
2021年6月18日 · Often not helpful in nonclassic cases Outlines architecture of liver and spleen To diagnose hemangiopericytoma, vascular smooth muscle, fibrosarcoma or fibrothecoma (stains each cell) vs. endothelial cell tumors (stains outside of all cells), MPNST (runs parallel to spindle tumor cells without surrounding them at the poles)
Pathology Outlines - Tuberculosis
6 天之前 · Acid fast stains, notably variations of the Ziehl-Neelsen and Kinyoun stains, are the stains of choice for demonstrating M. tuberculosis (Clin Microbiol Rev 2008;21:243) Fluorescent stains, including auramine-rhodamine, will also highlight acid fast bacilli (J Ayub Med Coll Abbottabad 2018;30:159, Henry Ford Hosp Med Bull 1962;10:287)
Pathology Outlines - Iron
2021年6月18日 · Perls method (Prussian blue stain): hydrochloric acid releases the protein bound to ferric iron, then potassium ferrocyanide binds with ferric iron to form ferric ferrocyanide, an insoluble blue compound Hemosiderin may be present in areas of old hemorrhage or be deposited in tissues with iron overload
Pathology Outlines - Stains & CD markers
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Pathology Outlines - Cytokeratin 34 beta E12
Comment: Immunohistochemical stain for CK34βE12 (HMWK) highlights the basal cells in all the cores and confirms the diagnosis. Additional references Urology Herald 2018;6:42 , J Med Case Rep 2010;4:186
Pathology Outlines - Intestinal metaplasia
2022年11月18日 · Alcian blue (AB) at pH 2.5: diagnostic stain but not routinely needed as gastric intestinal metaplasia can be readily identified in H&E; AB stains goblet cells bright blue due to the presence of sialomucins Periodic acid-Schiff Alcian blue (PAS AB): stains the acidic or sialomucins as purple and neutral mucins as magenta