Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids. The discovery and applications of clustered regularly interspaced short …

The CRISPR-Cas [clustered regularly interspaced short palindromic repeats and the CRISPR-associated genes (Cas)] is an adaptive immune system of prokaryotes against the invasion of …

Recent applications in bacteria have centered on multiplexed genome editing, programmable gene regulation, and sequence-specific antimicrobials, while future applications can build on …

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) technologies have greatly advanced our genetic engineering capabilities in the past few years. …

2024年2月12日 · To fully grasp CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technologies, it is important to understand where they come from. Importantly, these …

2021年4月15日 · Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids. The discovery and applications of clustered regularly …

2019年7月3日 · Using CRATES, we construct arrays for the single-effector nucleases Cas9, Cas12a, and Cas13a that mediated multiplexed DNA/RNA cleavage and gene regulation in …

Here, we present a novel, highly accurate, cost-, and time-saving strategy for CRISPR array assembly. Using this strategy, we efficiently assembled 12 CRISPR RNAs (crRNAs) (for …

4 天之前 · Figure 6 illustrates that CRISPR arrays are short sequences of repetitive DNA (repeats) interspersed with unique sequences (spacers) derived from viral or plasmid DNA that help …

Here, we show that CRISPR arrays utilized by the Cas9 nuclease can be shortened without compromising and sometimes even enhancing targeting activity. Using multiplexed gene …