2021年8月1日 · • Acute disease: virus detection (PCR, antigen) is most useful, serology of lower diagnostic value since most pigs die before antibody response (7-10 days) • Chronic or …
Development of a real-time PCR assay for detection of African …
Real-time PCR assays are highly sensitive, specific and rapid techniques for the identification of ASF virus (ASFV) (Section 3.8, OIE Terrestrial Manual, 2019). Although an ASFV p72 gene …
diagnostic tests for field application. A. frican swine fever (ASF) cannot be differentiated from other febrile haemorrhagic syndromes or bacterial septicaemias of pigs by either clinical or …
specific and sensitive detection of ASFV by real-time PCR at a comparably lower cost. In addition, a second real-time PCR test was developed and standardized, taking advantage of …
The protocol described below is a recommended real-time RT-PCR procedure for the detection of ASF virus (King assay). The RT step is not required for amplification of ASF viral DNA; …
hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Cl assical swine fever (CSF). The method uses two primer sets, …
Development of a novel real‐time PCR assay ... - Wiley Online …
Real-time PCR is the preferred first-line diagnostic for ASF. It is highly sensitive and specific, rapid and highly scalable (Fernández-Pinero et al., 2013 ; King et al., 2003 ). Several real-time PCR …
Development of Real-Time PCR Based on
2021年11月29日 · The real-time PCR assay developed in this study enables the detection of virus in the early stage of infection or low virus concentration, providing a good method for the early …
Molecular diagnosis of African Swine Fever by a new real-time PCR …
A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe …
Development and Validation of a Multiplex, Real-Time RT PCR …
2013年7月26日 · This study details the optimisation and evaluation of a novel, multiplex real time RT-PCR assay for the differential diagnosis of CSFV and/or ASFV alongside an exogenous …