pET-27b (+) pET27b质粒图谱、载体图谱、序列、价格、抗性、测 …
pET-27b载体带有N-pelB分泌信号肽标签，C-HSV标签和C-His标签。本载体与pet25b载体相比，只有抗性标记不同。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序 …
pET-27b(+) Sequence and Map - SnapGene
Bacterial vector with a signal sequence and a kanamycin resistance marker for expression of C-terminally HSV-tagged proteins in the periplasm. Sequence Author : MilliporeSigma (Novagen) …
pET-27b (+)_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研 …
The pET-27b (+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs …
Expression and Purification of Recombinant Proteins Using …
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under …
pET-27b(+) vector map and sequence - novoprolabs.com
The pET-27b(+) is a bacterial expression vector designed to produce C-terminally HSV-tagged proteins in the periplasm. It includes a signal sequence for proper protein targeting and a …
pET vector. Plasmid for Expression by T7 RNA Polymerase. | PPT
2020年2月9日 · The pET vector system is a powerful tool for expressing cloned genes in E. coli. It utilizes the strong T7 promoter to drive high-level expression of the gene when induced. The …
pET vector - | BDL・BioDynamics Laboratory Inc.
Vectors for protein expression in E. coli (T7 expression system). pET vector is an expression vector driven by T7 promoter. On the lineup of BDL, there are 3 types of vectors: high copy, …
The pET Expression System - Davidson
To start the process, your favorite gene (YFG) is cloned into a pET plasmid at the polylinker site. Both the T7 promoter and the lac operator are located 5' to YFG. When the T7 RNA …
pET Bacterial Recombinant Protein Vector - VectorBuilder
2025年1月10日 · The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system. Activation of expression is …
pET vectors | PPT - SlideShare
2018年1月5日 · The gene for T7 RNA polymerase (gene 1) is inserted into the chromosome of E. coli and transcribed from the lac promoter; therefore, it will be expressed only if the inducer …