Ex vivo lung perfusion (EVLP) is an innovative therapy applied to donor lungs outside of the body before transplantation that improves organ quality and makes lungs that were previously unsuitable safe for transplant.

目前，常规的用于生成 eVLP 的方法，一个是采用抗原与病毒与病毒衣壳蛋白共表达来生成 eVLP ，另外一个是Tag/Catcher 技术，基本原理是选择镶嵌 Catcher 序列的蛋白纳米颗粒作为骨架，例如，正八面体的 24 聚体铁蛋白（Ferritin）、正十二面体的 60 聚体 mi3 纳米颗粒 ...

2024年1月8日 · Here we report prime editor engineered virus-like particles (PE-eVLPs) that deliver prime editor proteins, prime editing guide RNAs and nicking single guide RNAs as transient ribonucleoprotein...

体外肺灌注 (ex vivo lung perfusion，EVLP)技术是在体外模拟肺通气及循环灌注状态对供肺进行评估和修复的工具，可以帮助改善供肺功能，使部分边缘供肺达到移植标准，从而增加边缘供肺的利用率。 过去全球只有20%~25%的供肺可以用于肺移植，但在EVLP技术应用于临床后，供肺利用率上升到了40%以上 [2]。 此外，EVLP技术还能显著降低原发移植物功能障碍发生率，提高肺移植受者术后生存率。 本文现就EVLP的定义和原理、供肺评估的技术方法、其在临床肺移植中的应 …

EVLP is a promising technology with the potential to address many of shortcomings of traditional organ donation. On-going research in the capabilities of EVLP to recondition injured lungs, expand preservation time, and reduce ischemia-reperfusion injury have made important strides towards achieving clinical translation.

Ex vivo lung perfusion (EVLP) is a rapidly developing and increasingly utilized technology to increase the lung donor pool for transplantation. EVLP has evolved into a device for logistical optimization of the lung transplant process and increases the …

Among potential alternatives to address this issue, ex vivo lung perfusion (EVLP) has emerged as a modern preservation technique that allows for more accurate lung assessment and also improvement of lung function. Its application in high-risk donor lungs has been successful and resulted in safe expansion of the donor pool.

2017年2月23日 · Ex vivo lung perfusion (EVLP) promises to be a comprehensive platform for assessment, reconditioning, and preservation of donor lungs and has been dramatically changing the face of clinical lung transplantation.

What is EVLP? EVLP is an innovative therapy. It is applied to donor lungs outside of the body before they are transplanted. It improves organ quality. It also makes lungs safe for transplant that used to be considered unsuitable. How does EVLP work? EVLP is used in lung transplantation.

2022年1月11日 · Methods to deliver gene editing agents in vivo as ribonucleoproteins could offer safety advantages over nucleic acid delivery approaches. We report the development and application of engineered DNA-free virus-like particles (eVLPs) that efficiently package and deliver base editor or Cas9 ribonucleoproteins.