Gain essential knowledge and practical tips to prevent cell culture contamination in your lab. Learn how to detect and treat common biological contaminants.

Culture contaminants may be biological or chemical, seen or unseen, destructive or seem-ingly benign, but in all cases they adversely affect both the use of your cell cultures and the quality of your research. Contamination problems can be divided into three classes:

Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or …

Monitoring cell cultures is an important part of everyday cell culture. This tutorial will help explain the basics of monitoring your cells, including what a healthy culture looks like, cell confluency, and the different phases of cell culture growth. This video will also show common indicators of bacterial and mycoplasma contamination.

2016年7月3日 · Be suspicious: Never trust that any culture is what it says on the label, even newly bought cells. Be cautious: Only buy from reputable cell banks. Quarantine incoming samples until their identity can be confirmed and their cleanliness status verified.

Contamination in Cell Culture: What to Do and How to Prevent It. Cell culture contamination remains one of the most significant challenges in laboratory research, potentially compromising experiment validity and reproducibility.

2022年11月1日 · Contamination is a constant risk in every cell culture laboratory. And while the threat of contamination will always remain, good working practices and proactive screening are essential to reduce the likelihood and severity of outbreaks—protecting your …