The nucleolus as a multiphase liquid condensate - Nature
2020年9月1日 · Recent studies have provided increasing support for the concept that the nucleolus represents a multilayered biomolecular condensate, whose formation by liquid–liquid phase separation (LLPS)...
The nucleolus functions as a phase-separated protein quality
2019年7月11日 · Here, we found that during stress, misfolded proteins enter the liquid-like GC phase of the nucleolus, where irreversible coaggregation of different misfolded protein species is prevented, allowing Hsp70-mediated extraction and refolding (or …
Recent studies have provided increasing support for the concept that the nucleolus represents a multilayered biomolecular condensate, whose formation by liquid–liquid phase separation (LLPS) facilitates the initial steps of ribosome biogenesis and other functions.
The nucleolus as a multiphase liquid condensate - PubMed
Recent studies have provided increasing support for the concept that the nucleolus represents a multilayered biomolecular condensate, whose formation by liquid-liquid phase separation (LLPS) facilitates the initial steps of ribosome biogenesis and other functions.
Birth of Nucleolar Compartments: Phase Separation-Driven
2019年12月5日 · The nucleolus is a multilayered biocondensate formed by liquid-liquid phase separation that adopts the behavior/biophysics of immiscible liquids (Feric et al., 2016). Put simply, it’s like when droplets of balsamic vinegar form in oil.
Phase Separation of Intrinsically Disordered Nucleolar Proteins …
2021年12月21日 · Phase separation of proteins that interact with nuclear lipids appear to form a pattern structure where each molecule occupies a defined position; therefore, the diffusion would follow a similar pattern as a spinodal decomposition.
Coexisting Liquid Phases Underlie Nucleolar Subcompartments
2016年6月16日 · The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles that appear to assemble through phase separation of their molecular components. However, many such RNP bodies contain internal subcompartments, and the mechanism of their formation remains unclear.
The Role of Liquid–Liquid Phase Separation in the Structure and ...
2023年9月30日 · In this chapter, we focus on liquid-like properties of nucleolus and describe how liquid–liquid phase separation of nucleolar proteins and rRNAs play a role in structural and functional dynamics of nucleolus. The nucleolus is the most prominent structure in the nucleus.
Evidence for and against Liquid-Liquid Phase Separation in the …
2019年11月1日 · The nucleus contains many different membraneless structures, including the nucleolus (orange), constitutive heterochromatin compartments (yellow), paraspeckles (green) and transcriptional condensates (blue), which have all been proposed to assemble through liquid-liquid phase separation (LLPS).
Birth of Nucleolar Compartments: Phase Separation-Driven Ribosomal …
2019年12月5日 · Phase separation relies on multivalent weak interactions between RNA and protein cell constituents. Importantly, these interactions are driven by function. Proteins with intrinsically disordered regions or low-complexity sequences, which are particularly abundant in the nucleolus, are directly involved in such interactions.