Concepts and practices used to develop functional PLGA-based ...
Derivatization of the surface of PLGA-based nanoparticulate carriers with cell-recognizable ligands: (A) physical association driven by the specific avidin-biotin binding affinity; (B–D) amide coupling reactions using carbodiimide reagents; (E) maleimide-thiol reaction; (F) thiol-thiol reaction; and (G) aldehyde-amine reaction. In most ...
Synthesis and Evaluation of BSA-Loaded PLGA–Chitosan …
2023年5月17日 · In spectra (Figure 2D,F), a new peak was observed at 1,688 and 1,686 cm –1, which indicated the amide I bond (−CONH−) formed by the chemical conjugation of CS onto PLGA NPs through coupling reaction.
Formulation of PLGA nano-carriers: specialized ... - RSC Publishing
This process is the most popular to conjugate the activated carboxyl group (–COOH) with the primary amine group by forming a very stable amide bond. The –COOH group of PLGA is activated by carbodiimide coupling agents (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and dicyclohexylcarbodiimide (DCC)) that produces an intermediate ...
PLGA protein nanocarriers with tailor-made ... - RSC Publishing
Subsequently, the three functional moieties with amine-reactive groups (fluram, Cy7.5, DFO) could be respectively integrated at the end of the PLGA molecule through coupling reactions . Further details are reported in the Experimental section.
2023年5月17日 · The composite NP were prepared by grafting CS on the surface of PLGA NPs through EDC-NHS coupling reaction. The size of bovine serum albumin (BSA)-loaded PLGA NPs and BSA-loaded PLGA−CS composite NPs was 288 ± 3 and 363 ± 4 nm, respectively. The zeta potential of PLGA NPs is −18 ± 0.23, and that of composite particles is 19 ± 0.40,
Formulation of stabilizer-free, nontoxic PLGA and elastin-PLGA ...
2021年3月15日 · Here, we developed a tunable, stabilizer-free PLGA nanoparticle formulation capable of encapsulating plasmid DNA and demonstrated the formation of an elastin-like polymer PLGA hybrid nanoparticle with exceptional stability and biocompatibility.
Gemcitabine-loaded PLGA-PEG immunonanoparticles for …
2013年9月24日 · The PLGA-PEG-NH 2 diblock copolymer was synthesized by coupling reaction via amide linkage between PEG-diamine and activated PLGA. PLGA and PLGA-PEG-NH 2 nanoparticles loaded with gemcitabine were prepared using the double-emulsion solvent evaporation method.
Designing theranostic nanocarriers with high protein payload and multimodality tracking without cross interferences between the different imaging probes and the delicate protein cargo is challenging. Here, chemical modications of poly(lactic- co-glycolic acid) (PLGA) to produce nanocapsules (NCs) that incor-
Oriented attachment of VNAR proteins, via site-selective …
2019年7月7日 · Here, amine groups distributed throughout native V NAR E4 6 (i.e. containing no PD 5 linker) were reacted with NHS esters on the surface of PLGA–PEG–NHS NP 9, with coupling proceeding via amide bond formation to generate a nanoconjugate of 197.8 ± 5.1 nm in size (random lysine modified E4 NP 11).
Synthesis of L-Ornithine- and L-Glutamine-Linked PLGAs as
2023年10月5日 · L-ornithine or L-glutamine was integrated into a PLGA polymer with EDC coupling reactions as a structure developer after the synthesis of PLGA via the polycondensation and ring-opening polymerization of lactide and glycolide.