2020年9月24日 · With the long-term goal of understanding chloroplast proteome dynamics in response to stress, we describe a high-yielding method to isolate intact tomato chloroplasts …

Here we describe a simple, rapid, and low-cost method for isolating a high yield of Arabidopsis chloroplasts. The method can readily be applied to wild-type plants and different mutants, and …

2006年12月14日 · This method has been developed to yield highly purified intact chloroplasts from Chlamydomonas reinhardtii. This procedure involves breaking cell-wall-deficient cells by …

Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting …

1991年12月1日 · This procedure generates relatively high yields of chloroplasts capable of CO 2 fixation. These chloroplasts were characterized by electron microscopy, marker enzyme …

The isolation of the photosynthetically competent chloroplast preparations was undertaken by means of the density gradient centrifugation on the modified silica sol “Percoll.” A clear …

at 400x under microscope to determine composition and illustrate in notebook. Note appearance of components and degree of heterogeneity. (Label cells, ghosts, chloro plasts, mitochondria, …

2010年1月1日 · Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium …

2024年3月20日 · This chapter describes procedures to isolate intact chloroplasts and their three main compartments: the stroma, thylakoids, and the bounding envelope. The method is based …

a crude chloroplast pellet from a single differential centrifugation spin (see methods) was layered onto a density gradient. Compared to Figures 1 and 2, the elution profiles in Figure 3 differed …