The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome ... of toxicity and higher knock-in efficiency Comparing ...

However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient. Yet, inserting DNA is crucial to engineering CAR T cells.

Genome editing with various CRISPR-Cas molecule ... a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology-directed repair.

Both gene knockout and knock-in models are powerful ... Nuclease-induced DNA DSBs can be repaired through either ...