TGEM(0.1): (0.1 refers to the NaCl concentration) 20 mM Tris-HCl (pH 7.9), 20% glycerol, 1 mM EDTA, 5 mM MgCl 2 ... background in the interaction assay. Distribute 40 μl of resin slurry (10 ...

Incubate at 37 °C overnight. MNase stop buffer consists of 100 mM EDTA and 10 mM EGTA (pH 7.5). 8| Extract samples with 200 μl of phenol/chloroform and gently mix by flicking or rocking the tubes.

KRAS enzyme titration with varying EDTA concentrations using the Transcreener GDP FP Assay. Assay conditions include 2 µM GTP, 20 mM Tris pH 7.5, 50 mM NaCl, 5 mM MgCl 2, 0.1 mg/mL BSA ...

RNA from EDTA blood was, however ... In order to distinguish results below the detection limit of an assay from missing data, the former were replaced with the detection limit divided by two.