Initial qPCR cycles with minimal amplification and relatively low fluorescent signals form a baseline for analysis. At a specific cycle number, known as the threshold cycle (Ct), the fluorescent ...

In studies of gene expression levels, technologies such as DNA microarrays and real-time quantitative RT-PCR (qPCR) have essentially replaced traditional approaches such as northern blot analysis ...

The UltraMarathonRT ® RT-qPCR Kit dramatically improves data reliability in quantitative PCR by maintaining consistent cycle ...