After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here 1 uses ...

1. Prepare a polyacrylamide minigel of the desired concentration containing 8 M urea (for example, Bio-Rad Mini-Protean). 2. While the gel is polymerizing, mix the following reagents: Incubate the ...