SMLM can achieve resolutions down to 10-20 nanometers, surpassing STED. However, SMLM typically requires longer acquisition times and specialized fluorophores, and is more challenging for live-cell ...

We show this by performing continuous TauSTED imaging of a sample of Cos7 cells immunostained for proteins forming the nuclear pore basket (Fig. 2f) at extremely low STED light dose (5%).

Leica Microsystems now sells an STED microscope with a resolution of 80 nm. At present, the far-field methods are those chosen for imaging structures below a surface, such as inside a cell.

Inspired by a super-resolution microscopy technique known as STED, we are showing that it is indeed possible to write features whose dimensions are substantially smaller than the diffraction limit.

However, interesting processes occur at a length scale below this limit, particularly in biological cells. STED microscopy overcomes this barrier, achieving resolution up to 10 times better than ...

Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary. The images show a STED and AFM experiment of living A549 cells imaged at 37 °C in medium. (1) STED image of ...