This study introduces smRandom-seq2, a high-throughput and high-resolution single-microbe RNA sequencing method, which ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

The inserts were sequenced using primers matching the vector sequence flanking the genomic DNA insert, and overlapping shotgun clones were used to generate a DNA sequence spanning the entire BAC ...

The pSM2 plasmid harbors a conditional bacterial origin of replication ... 3–10,000 shRNAs). The sequence of the U6 sequencing primer (beginning at −42 from the start of transcription) is ...

Bacteria producing extended-spectrum beta-lactamases (ESBL) and plasmidic AmpC (pAmpC) as well as carbapenemases were screened. Whole genome sequencing was used to determine resistance and virulence ...

while offering a series of advantages such as the ability to analyze large amounts of samples rapidly and to perform high-quality sequencing of total bacterial DNA. Marcel de Zoete concludes: ...

As the primary diagnostic tool for infectious diseases, traditional culture techniques are coming under scrutiny for failing to universally detect infections resulting from biofilms, viable but ...