The chemiluminescence is measured to estimate HBV DNA. [30] Real-time PCR In real-time PCR, the HBV DNA in the sample is amplified in a non-nested PCR in the presence of specific TaqMan probes or ...

Real-time PCR is the favored method for measuring gene expression. Researchers benefit from a vast and growing choice of reagents and instruments for their experiments. Laura Bonetta reports.

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.