Incubate at 37 °C overnight. MNase stop buffer consists of 100 mM EDTA and 10 mM EGTA (pH 7.5). 8| Extract samples with 200 μl of phenol/chloroform and gently mix by flicking or rocking the tubes.

TGEM(0.1): (0.1 refers to the NaCl concentration) 20 mM Tris-HCl (pH 7.9), 20% glycerol, 1 mM EDTA, 5 mM MgCl 2 ... background in the interaction assay. Distribute 40 μl of resin slurry (10 ...