透明质酸（HA）在多领域应用广泛，传统生产方式存在局限。研究人员开展蓝藻代谢工程研究，利用模块化基因表达和 CRISPRi 系统优化代谢途径。结果显示，工程菌株 SeHA226 可高效合成 HA，为大规模光合生产 HA 提供方向。 在生物医学和化妆品领域，透明质酸 ...

为探究噬菌体基因功能，美国加州大学伯克利分校的研究人员开展 CRISPRi-ART 技术研究，发现该技术可用于多种噬菌体功能基因组分析，揭示超 90 个未知功能基因，为噬菌体研究提供新平台。 噬菌体，作为地球上数量最为庞大且遗传多样性极高的生物群体 ...

CRISPR interference (CRISPRi) is a novel method for specific gene knockdown. It's CRISPR, without the cut. The Horizon CRISPRi system uses a deactivated Cas9 nuclease (dCas9) fused to our proprietary ...

We provide access to CRISPR KO (knockout), CRISPRi (interference) and CRISPRa (activation) technologies, which change the phenotype of cells by eliminating, reducing or increasing gene expression.

More information: XinYue Wang et al, Whole genome CRISPRi screening identifies druggable vulnerabilities in an isoniazid resistant strain of Mycobacterium tuberculosis, Nature Communications (2024).

DOI: 10.1038/s41467-024-53933-8 XinYue Wang et al, Whole genome CRISPRi screening identifies druggable vulnerabilities in an isoniazid resistant strain of Mycobacterium tuberculosis, Nature ...