为提升 HDR 相关基因编辑效率，研究人员筛选化合物，发现 HDAC 抑制剂有效，为基因编辑提供新策略。 基因编辑技术近年来在生命科学领域掀起了一阵 “变革之风”，其中 CRISPR-Cas9 系统凭借其独特优势备受瞩目。CRISPR-Cas9（成簇规律间隔短回文重复序列 / CRISPR ...

这就不得不提到，2018年加州大学旧金山分校（UCSF）研究团队在《自然》杂志上发表文章，Alexander Marson教授和他的同事揭示了一种高效的T细胞改造方法，通过共电穿孔的方式将Cas9-gRNA核糖核蛋白（RNP）复合物与双链DNA(dsDNA)或单链DNA(ssDNA) HDR模板共同导入至目标 ...

Danielle is a Senior Editor at The Scientist. She has a background in neuroscience. Her science communication experience spans journalism, scientific publishing, and science education and outreach.

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

View Full Profile. Learn about our Editorial Policies. “We have found that Cas9-mediated HDR frequencies can be increased by rationally designing the orientation, polarity and length of the donor ...

In cystic fibrosis, researchers have used CRISPR HDR-mediated knock-in to correct mutations in the CFTR gene in airway stem ...

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...